Jose Rod R, Rodriguez Maria J, Dixon Thomas A, Omenetto Fiorenzo, Kaplan David L
Department of Biomedical Engineering, 4 Colby Street, Tufts University, Medford, Massachusetts 02155, United States.
ACS Biomater Sci Eng. 2016 Oct 10;2(10):1662-1678. doi: 10.1021/acsbiomaterials.6b00088. Epub 2016 Apr 7.
3D printing is an additive manufacturing (AM) technique that has quickly disrupted traditional design and manufacturing strategies. New structures can be manufactured that could not be fabricated using other methods. These new capabilities are considered by many to hallmark a historic shift representative of a new industrial revolution. Exciting utilities of this evolving technology are the fields of biomedical engineering and translational medicine, particularly in applying three-dimensional (3D) printing toward enabling on-demand fabrication of customized tissue scaffolds and medical device geometries. AM techniques are promising a future where on-demand production of patient-specific living tissues is a reality. In this review, we cover the rapid evolution and widespread concepts of a bio-"ink" and bioprinted devices and tissues from the past two decades as well as review the various additive manufacturing methods that have been used toward 3D bioprinting of cells and scaffolds with a special look at the benefits and practical considerations for each method. Despite being a young technology, the evolution and impact of AM in the fields of tissue engineering and regenerative medicine has progressed rapidly. We finish the review by looking toward the future of bioprinting and identify some of the current bottlenecks facing the blossoming industry.
3D打印是一种增材制造（AM）技术，它迅速颠覆了传统的设计和制造策略。可以制造出用其他方法无法制造的新结构。许多人认为这些新能力标志着一场具有历史意义的转变，代表着一场新的工业革命。这项不断发展的技术令人兴奋的应用领域是生物医学工程和转化医学，特别是在将三维（3D）打印用于按需制造定制的组织支架和医疗设备几何形状方面。增材制造技术有望实现一个按需生产患者特异性活组织的未来。在这篇综述中，我们涵盖了过去二十年生物“墨水”以及生物打印设备和组织的快速发展和广泛概念，并回顾了用于细胞和支架的3D生物打印的各种增材制造方法，特别关注每种方法的优点和实际考虑因素。尽管3D打印是一项新兴技术，但增材制造在组织工程和再生医学领域的发展和影响进展迅速。我们通过展望生物打印的未来来结束这篇综述，并确定这个蓬勃发展的行业目前面临的一些瓶颈。
