Helling A L, Tsekoura E K, Biggs M, Bayon Y, Pandit A, Zeugolis D I
Sofradim Production, A Medtronic Company, Trévoux, France.
ACS Biomater Sci Eng. 2017 Sep 11;3(9):1922-1932. doi: 10.1021/acsbiomaterials.5b00563. Epub 2016 Apr 27.
Matrix metalloproteinase-1 and -8 are active during the wound healing and remodelling processes, degrading native extracellular matrix and implantable devices. However, traditional in vitro assays utilize primarily matrix metalloproteinase-1 to mimic the in vivo degradation microenvironment. Herein, we assessed the influence of various concentrations of matrix metalloproteinase- 1 and 8 (50, 100, and 200 U/mL) as a function of pH (5.5 and 7.4) and time (3, 6, 9, 12, and 24 h) on the degradation profile of three tissue grafts (chemically cross-linked Permacol, nonchemically cross-linked Permacol and nonchemically cross-linked Strattice) and a collagen biomaterial (nonchemically cross-linked collagen sponge). Chemically cross-linked and nonchemically cross-linked Permacol samples exhibited the highest resistance to enzymatic degradation, while nonchemically cross-linked collagen sponges exhibited the least resistance to enzymatic degradation. Qualitative and quantitative degradation analysis of all samples revealed a similar degradation profile over time, independently of the matrix metalloproteinase used and its respective concentration and pH. These data indicate that matrix metalloproteinase-1 and matrix metalloproteinase-8 exhibit similar degradation profile in vitro, suggesting that matrix metalloproteinase-8 should be used for collagenase assay.
基质金属蛋白酶-1和-8在伤口愈合和重塑过程中具有活性，可降解天然细胞外基质和可植入装置。然而，传统的体外试验主要利用基质金属蛋白酶-1来模拟体内降解微环境。在此，我们评估了不同浓度的基质金属蛋白酶-1和-8（50、100和200 U/mL）作为pH值（5.5和7.4）和时间（3、6、9、12和24小时）的函数对三种组织移植物（化学交联的Permacol、非化学交联的Permacol和非化学交联的Strattice）以及一种胶原蛋白生物材料（非化学交联的胶原海绵）降解情况的影响。化学交联和非化学交联的Permacol样品对酶降解的抵抗力最强，而非化学交联的胶原海绵对酶降解的抵抗力最弱。所有样品的定性和定量降解分析显示，随着时间的推移，降解情况相似，与所使用的基质金属蛋白酶及其各自的浓度和pH值无关。这些数据表明，基质金属蛋白酶-1和基质金属蛋白酶-8在体外表现出相似降解情况，这表明基质金属蛋白酶-8应用于胶原酶测定。
