Zhou Zi-Fei, Zhang Fan, Wang Jian-Guang, Chen Quan-Chi, Yang Wei-Zhi, He Ning, Jiang Ying-Ying, Chen Feng, Liu Jun-Jian
Department of Orthopedic Surgery, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
Department of Orthopedic Surgery, Shanghai East Hospital, Tongji University, Shanghai 200072, China.
ACS Biomater Sci Eng. 2016 Sep 12;2(9):1572-1581. doi: 10.1021/acsbiomaterials.6b00335. Epub 2016 Aug 10.
Peripheral nerve injuries represent a great challenge for surgeons. The conductive neural scaffold has experienced increasing interest because of its good biocompatibility and similar electrical properties as compared to those of a normal nerve. Herein, nerve conduits made from poly(d,l-lactide)--poly(ethylene glycol) and polypyrrole (20%, 30%, and 50%) (PELA-PPY) were prepared by electrospinning, and used in regeneration of peripheral nerve defects. The results of an experiment indicated a high biocompatibility for the as-prepared materials, supporting the attachment and proliferation of a rat pheochromocytoma PC-12 cell. Furthermore, the PELA-PPY nerve conduit implanted in the sciatic nerve defects (10 mm) of the Spraguee-Dawley rats for 12 weeks showed similar results with the autograft, while it demonstrated a better outcome than the PELA nerve conduit in electrophysiological examination, sciatic function index, total amount of regenerated myelinated nerve fibers, axon diameter, myelin thickness, and several immunohistochemistry indices (S-100, laminin, neurofilament, bromodeoxyuridine, and glial fibrillary acidic portein). We supposed that the bioactivity is mainly generated by the PPY in composite nanofibers which could transmit self-originated electrical stimulation between cells. Due to the facile preparation and excellent performance, the PPY-PELA nerve conduit is promising for use as a bioengineered biomaterial for peripheral nerve regeneration.
周围神经损伤对外科医生来说是一个巨大的挑战。导电神经支架因其良好的生物相容性以及与正常神经相似的电学特性而越来越受到关注。在此，通过静电纺丝制备了由聚（d，l-丙交酯）-聚（乙二醇）和聚吡咯（20%、30%和50%）（PELA-PPY）制成的神经导管，并将其用于周围神经缺损的再生。实验结果表明所制备的材料具有高生物相容性，支持大鼠嗜铬细胞瘤PC-12细胞的附着和增殖。此外，将PELA-PPY神经导管植入Sprague-Dawley大鼠的坐骨神经缺损（10毫米）中12周，其结果与自体移植相似，而在电生理检查、坐骨神经功能指数、再生有髓神经纤维总量、轴突直径、髓鞘厚度以及几个免疫组织化学指标（S-100、层粘连蛋白、神经丝、溴脱氧尿苷和胶质纤维酸性蛋白）方面，它比PELA神经导管表现出更好的结果。我们推测生物活性主要由复合纳米纤维中的聚吡咯产生，其可以在细胞之间传递自身产生的电刺激。由于制备简便且性能优异，PPY-PELA神经导管有望用作周围神经再生的生物工程生物材料。
