State Key Lab for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Department of Orthopaedic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:190-199. doi: 10.1016/j.msec.2018.09.021. Epub 2018 Sep 8.
According to our previous study, polypyrrole (Ppy) possessed good conductivity and non-cytotoxicity. In this study, the surface of electrospun poly(l-lactic acid-co-ε-caprolactone)/silk fibroin (PLCL/SF) was coated with Ppy to fabricate Ppy-coated nerve guidance conduit (NGC). Firstly, the presence of Ppy on the prepared NGC was characterized and confirmed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopic spectra (XPS) and Fourier transforms infrared spectroscopy (FTIR). Subsequently, Ppy-coated NGC was used to repair a 10 mm sciatic nerve gap in vivo. 4 and 12 weeks after implantation, the regenerated nerve tissues on defect sites were removed and sectioned for further evaluation. Histological analysis with hematoxylin-eosin (HE), toluidine blue (TB), and luxol fast blue (LFB) staining indicated that the coated Ppy could promote SCs proliferation in early post-surgery, and enhance myelin formation in later post-surgery. In consideration of immunofluorescence and morphology observation with SEM and TEM, it showed that the nerve regeneration of Ppy-coated NGC group was close to autograft group, which was better than PLCL/SF NGC. In addition, walking track analysis indicated that Ppy-coated NGC group showed a similar performance compared with the autograft group, and significantly better than PLCL/SF NGC group. These promising results showed the potential of Ppy-coated NGC in peripheral nerve regeneration.
根据我们之前的研究，聚吡咯（Ppy）具有良好的导电性和非细胞毒性。在本研究中，我们将电纺聚（L-丙交酯-共-ε-己内酯）/丝素纤维（PLCL/SF）表面涂覆聚吡咯，以制备聚吡咯涂层神经导管（NGC）。首先，通过扫描电子显微镜（SEM）、X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FTIR）对制备的 NGC 上 Ppy 的存在进行了表征和确认。随后，将 Ppy 涂层 NGC 用于体内修复 10mm 坐骨神经间隙。植入后 4 和 12 周，从缺损部位取出再生神经组织并进行切片以进一步评估。苏木精-伊红（HE）、甲苯胺蓝（TB）和卢夫快速蓝（LFB）染色的组织学分析表明，涂层 Ppy 可以促进术后早期SCs 的增殖，并增强术后晚期髓鞘的形成。考虑到免疫荧光和 SEM、TEM 的形态观察，结果表明 Ppy 涂层 NGC 组的神经再生接近自体移植物组，优于 PLCL/SF NGC 组。此外，行走轨迹分析表明，Ppy 涂层 NGC 组与自体移植物组表现相似，明显优于 PLCL/SF NGC 组。这些有前景的结果表明 Ppy 涂层 NGC 在周围神经再生方面具有潜力。
