Son Sung-Hun, Lee Mi-Kyung, Son Ye-Eun, Park Hee-Soo
School of Food Science and Biotechnology Kyungpook National University, Daegu 41566, Korea.
Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 56212, Korea.
Microorganisms. 2021 Jan 11;9(1):144. doi: 10.3390/microorganisms9010144.
Homeobox transcription factors are conserved in eukaryotes and act as multi-functional transcription factors in filamentous fungi. Previously, it was demonstrated that HbxB governs fungal development and spore viability in . Here, the role of HbxB in was further characterized. RNA-sequencing revealed that HbxB affects the transcriptomic levels of genes associated with trehalose biosynthesis and response to thermal, oxidative, and radiation stresses in asexual spores called conidia. A phenotypic analysis found that deletion mutant conidia were more sensitive to ultraviolet stress. The loss of increased the mRNA expression of genes associated with β-glucan degradation and decreased the amount of β-glucan in conidia. In addition, deletion affected the expression of the sterigmatocystin gene cluster and the amount of sterigmatocystin. Overall, these results indicated that HbxB is a key transcription factor regulating trehalose biosynthesis, stress tolerance, β-glucan degradation, and sterigmatocystin production in conidia.
同源异型盒转录因子在真核生物中保守，并在丝状真菌中作为多功能转录因子发挥作用。此前，已证明HbxB在[具体真菌名称未给出]中调控真菌发育和孢子活力。在此，进一步表征了HbxB在[具体真菌名称未给出]中的作用。RNA测序显示，HbxB影响与海藻糖生物合成以及在无性孢子（称为分生孢子）中对热、氧化和辐射应激反应相关基因的转录组水平。表型分析发现，[具体真菌名称未给出]缺失突变体分生孢子对紫外线应激更敏感。[具体真菌名称未给出]的缺失增加了与β-葡聚糖降解相关基因的mRNA表达，并降低了分生孢子中β-葡聚糖的含量。此外，[具体真菌名称未给出]的缺失影响了柄曲霉素基因簇的表达和柄曲霉素的产量。总体而言，这些结果表明HbxB是调控[具体真菌名称未给出]分生孢子中海藻糖生物合成、应激耐受性、β-葡聚糖降解和柄曲霉素产生的关键转录因子。
