Division of Hematology, Brigham and Women's Hospital, Boston, MA. Department of Medicine, Harvard Medical School, Boston, MA. Broad Institute of MIT and Harvard, Cambridge, MA.
Arterioscler Thromb Vasc Biol. 2021 Mar;41(3):1012-1018. doi: 10.1161/ATVBAHA.120.314654. Epub 2021 Jan 14.
The blood system is often represented as a tree-like structure with stem cells that give rise to mature blood cell types through a series of demarcated steps. Although this representation has served as a model of hierarchical tissue organization for decades, single-cell technologies are shedding new light on the abundance of cell type intermediates and the molecular mechanisms that ensure balanced replenishment of differentiated cells. In this Brief Review, we exemplify new insights into blood cell differentiation generated by single-cell RNA sequencing, summarize considerations for the application of this technology, and highlight innovations that are leading the way to understand hematopoiesis at the resolution of single cells. Graphic Abstract: A graphic abstract is available for this article.
血液系统通常表现为一种树状结构，干细胞通过一系列明确的步骤产生成熟的血细胞类型。尽管这种表示形式作为分层组织的模型已经有几十年了，单细胞技术正在为中间细胞类型的丰富性和确保分化细胞平衡补充的分子机制提供新的认识。在这篇简短的综述中，我们举例说明了单细胞 RNA 测序产生的对血细胞分化的新见解，总结了应用这项技术的注意事项，并强调了引领单细胞水平理解造血的创新。
