Department of Family Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.
Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China.
Eur J Med Res. 2021 Jan 13;26(1):7. doi: 10.1186/s40001-021-00479-w.
The aim of this study was to detect the expression of interleukin (IL)-1β and transforming growth factor (TGF)-β1 in the colonic tissue and serum of irritable bowel syndrome (IBS) rats, as well as the distribution and expression of corticotropin-releasing factor (CRF) in the spinal cord and brain of the visceral hypersensitivity rats, thus to ascertain the mechanism of visceral hypersensitivity signal conduction pathway.
The expression of IL-1β and TGF-β1 in the colonic tissue and serum of IBS rats was screened by the liquid chip technology and verified by RT-PCR technology. Then the quantitative analysis of CRF in the spinal cord and brain was achieved by the immunohistochemical method and computerized image system.
The rat model with visceral hypersensitivity was successfully established. Among the screened indicators of IL-1β and TGF-β1 in colon tissue and serum, only the expression of IL-1β in the model group was up-regulated (P < 0.05). The immunohistochemical method showed that CRF was expressed in the spinal cord, hypothalamus, and the third ventricle. The positive index number of the model groups was higher than that of the control group (P < 0.01).
From the research, it can be inferred that IL-1β may participate in the pathogenesis mechanism of IBS via regulating the colon function. The increasing expression of CRF linked to stress in the spinal cord, hypothalamus and the third ventricle indicated that it might play an important role in the mechanisms of visceral hypersensitivity signal conduction pathway.
本研究旨在检测肠易激综合征（IBS）大鼠结肠组织和血清中白细胞介素（IL）-1β和转化生长因子（TGF）-β1的表达，以及内脏高敏感大鼠脊髓和脑内促肾上腺皮质释放因子（CRF）的分布和表达，以确定内脏高敏感信号传导通路的机制。
采用液相芯片技术筛选 IBS 大鼠结肠组织和血清中 IL-1β和 TGF-β1的表达，并通过 RT-PCR 技术进行验证。然后采用免疫组织化学法和计算机图像系统对脊髓和脑内 CRF 进行定量分析。
成功建立了内脏高敏感大鼠模型。在筛选的结肠组织和血清中 IL-1β和 TGF-β1的指标中，只有模型组的 IL-1β表达上调（P < 0.05）。免疫组织化学法显示 CRF 在脊髓、下丘脑和第三脑室表达。模型组的阳性指数高于对照组（P < 0.01）。
从研究中可以推断，IL-1β可能通过调节结肠功能参与 IBS 的发病机制。脊髓、下丘脑和第三脑室中与应激相关的 CRF 表达增加表明其可能在内脏高敏感信号传导通路的机制中发挥重要作用。
