Nemoto Akira, Goyagi Toru
Department of Anesthesia and Intensive Care Medicine, Akita University Graduate School of Medicine, 1-1-1, Hondo, Akita, 010-0843, Japan.
Lab Anim Res. 2021 Jan 13;37(1):7. doi: 10.1186/s42826-020-00083-9.
Sarcopenia promotes skeletal muscle atrophy and exhibits a high mortality rate. Its elucidation is of the highest clinical importance, but an animal experimental model remains controversial. In this study, we investigated a simple method for studying sarcopenia in rats.
Muscle atrophy was investigated in 24-week-old, male, tail-suspended (TS), Sprague Dawley and spontaneously hypertensive rats (SHR). Age-matched SD rats were used as a control group. The skeletal muscle mass weight, muscle contraction, whole body tension (WBT), cross-sectional area (CSA), and Muscle RING finger-1 (MuRF-1) were assessed. Enzyme-linked immunosorbent assay was used to evaluate the MuRF-1 levels. Two muscles, the extensor digitorum longus and soleus muscles, were selected for representing fast and slow muscles, respectively. All data, except CSA, were analyzed by a one-way analysis of variance, whereas CSA was analyzed using the Kruskal-Wallis test. Muscle mass weight, muscle contraction, WBT, and CSA were significantly lower in the SHR (n = 7) and TS (n = 7) groups than in the control group, whereas MuRF-1 expression was dominant.
TS and SHR presented sarcopenic phenotypes in terms of muscle mass, muscle contraction and CSA. TS is a useful technique for providing muscle mass atrophy and weakness in an experimental model of sarcopenia in rats.
肌肉减少症会促进骨骼肌萎缩，且死亡率很高。对其进行阐释具有极其重要的临床意义，但动物实验模型仍存在争议。在本研究中，我们探究了一种在大鼠中研究肌肉减少症的简单方法。
对24周龄的雄性尾悬吊（TS）的斯普拉格-道利大鼠和自发性高血压大鼠（SHR）的肌肉萎缩情况进行了研究。将年龄匹配的SD大鼠用作对照组。评估了骨骼肌质量重量、肌肉收缩、全身张力（WBT）、横截面积（CSA）和肌肉环状指蛋白-1（MuRF-1）。采用酶联免疫吸附测定法评估MuRF-1水平。分别选取趾长伸肌和比目鱼肌这两块肌肉来代表快肌和慢肌。除CSA外，所有数据均采用单因素方差分析进行分析，而CSA则采用Kruskal-Wallis检验进行分析。SHR组（n = 7）和TS组（n = 7）的肌肉质量重量、肌肉收缩、WBT和CSA均显著低于对照组，而MuRF-1表达占主导。
TS和SHR在肌肉质量、肌肉收缩和CSA方面呈现出肌肉减少症的表型。TS是一种在大鼠肌肉减少症实验模型中造成肌肉质量萎缩和无力的有用技术。
