Sharifi Sina, Islam Mohammad Mirazul, Sharifi Hannah, Islam Rakibul, Nilsson Per H, Dohlman Claes H, Mollnes Tom Eirik, Paschalis Eleftherios I, Chodosh James
Disruptive Technology Laboratory and Schepens Eye Research Institute, Massachusetts Eye and Ear; Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
Department of Immunology, Oslo University Hospital, Rikshospitalet, University of Oslo, Oslo, Norway.
Transl Vis Sci Technol. 2020 Dec 23;9(13):41. doi: 10.1167/tvst.9.13.41. eCollection 2020 Dec.
To evaluate titanium (Ti) sputtering of the poly(methyl methacrylate) (PMMA) stem of the Boston Keratoprosthesis (BK) as a method to enhance interfacial adhesion between the PMMA and the recipient corneal tissue.
PMMA specimens were plasma treated with Ar/O and coated with Ti using a DC magnetron sputtering instrument. The topography and hydrophilicity of the surfaces were characterized using atomic force microscopy and a water contact angle instrument, respectively. Scratch hardness and adhesion of the Ti film were measured using a mechanical tester. Biocompatibility assessments were performed using cultured human corneal fibroblasts and whole blood ex vivo. The optical quality of the Ti sputtered BK was evaluated using a custom-made optical bench.
By contact angle studies, the Ti coating improved PMMA hydrophilicity to match that of medical-grade Ti (Ti-6Al-4V-ELI). Ti sputtering of contact surfaces resulted in a plate-like morphology with increased surface roughness, without impacting the transparency of the BK optical component. Scratch testing indicated that the mechanical behavior of the Ti coating was similar to that of casted Ti, and the coating was stable in pull-off adhesion testing. Sputtered Ti film was highly biocompatible based on tests of cell viability, adhesion, proliferation, differentiation, collagen deposition, and keratocan expression, the properties of which exceeded those of uncoated PMMA and did not induce increased complement activation.
Titanium coating of the BK stem generated a mechanically and biologically favorable interface, which may help to enhance corneal stromal adhesion and biocompatibility.
Improving the biocompatibility of the BK PMMA stem may improve long-term outcomes of implantation.
评估波士顿人工角膜（BK）聚甲基丙烯酸甲酯（PMMA）柄的钛（Ti）溅射，作为增强PMMA与受体角膜组织之间界面粘附的一种方法。
使用直流磁控溅射仪对PMMA标本进行Ar/O等离子体处理并涂覆Ti。分别使用原子力显微镜和水接触角仪对表面的形貌和亲水性进行表征。使用机械测试仪测量Ti膜的划痕硬度和附着力。使用培养的人角膜成纤维细胞和全血进行体外生物相容性评估。使用定制的光具座评估Ti溅射BK的光学质量。
通过接触角研究，Ti涂层改善了PMMA的亲水性，使其与医用级Ti（Ti-6Al-4V-ELI）相匹配。接触表面的Ti溅射产生了具有增加的表面粗糙度的板状形态，而不会影响BK光学组件的透明度。划痕测试表明，Ti涂层的机械性能与铸造Ti相似，并且该涂层在剥离附着力测试中稳定。基于细胞活力、粘附、增殖、分化、胶原蛋白沉积和角膜蛋白表达的测试，溅射的Ti膜具有高度生物相容性，其性能超过未涂层的PMMA，并且不会诱导补体激活增加。
BK柄的钛涂层产生了机械和生物学上有利的界面，这可能有助于增强角膜基质粘附和生物相容性。
改善BK PMMA柄的生物相容性可能会改善植入的长期效果。
