Huang Si, Fu Yu, Mo Anchun
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.
Department of Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Front Chem. 2022 Sep 29;10:991481. doi: 10.3389/fchem.2022.991481. eCollection 2022.
Two-dimensional(2D)MXenes have continued to receive increasing interest from researchers due to their graphene-like properties, in addition to their versatile properties for applications in electronic devices, power generation, sensors, drug delivery, and biomedicine. However, their construction and biological properties as titanium coatings to prevent peri-implantitis are still unclear. In this work, few-layer TiCT MXene coatings with different thicknesses at varied depositing voltages (30, 40, and 50 V) were constructed by anodic electrophoretic deposition without adding any electrolytic ions. cytocompatibility assay was performed on preosteoblasts (MC3T3-E1) cell lines after the characterization of the coating. Meanwhile, the antibacterial activity against bacteria which are closely related to peri-implantitis including and its drug-resistant strain was further investigated. MXene-coated titanium models with different thicknesses were successfully assembled by analyzing the results of characterization. The compounding of TiCT could significantly improve the initial adhesion and proliferation of MC3T3-E1 cells. Moreover, the coating can effectively inhibit the adhesion and cell activity of and , and expressed greater restricting behavior than . The ability to promote antibacterial activity is proportional to the content of TiCT. Its antioxidant capacity to reduce ROS in the culture environment and bacterial cells was first revealed. In summary, this work shows a new avenue for MXene-based nano-biomaterials under the clinical problem of multiple antibiotic resistance.
二维（2D）MXenes因其类石墨烯特性，以及在电子设备、发电、传感器、药物递送和生物医学等应用中的多功能特性，一直受到研究人员越来越多的关注。然而，它们作为预防种植体周围炎的钛涂层的结构和生物学特性仍不清楚。在这项工作中，通过阳极电泳沉积法，在不添加任何电解离子的情况下，构建了不同厚度（在30、40和50 V不同沉积电压下）的少层TiCT MXene涂层。在对涂层进行表征后，对前成骨细胞（MC3T3-E1）细胞系进行了细胞相容性测定。同时，进一步研究了针对与种植体周围炎密切相关的细菌及其耐药菌株的抗菌活性。通过分析表征结果，成功组装了不同厚度的MXene涂层钛模型。TiCT的复合可以显著提高MC3T3-E1细胞的初始黏附及增殖能力。此外，该涂层能有效抑制牙龈卟啉单胞菌和具核梭杆菌的黏附及细胞活性，且TiCT表现出比具核梭杆菌更强的抑制作用。促进抗菌活性的能力与TiCT的含量成正比。首次揭示了其在培养环境和细菌细胞中降低活性氧的抗氧化能力。总之，这项工作为多重耐药性临床问题下基于MXene的纳米生物材料开辟了一条新途径。
