Yao Lei, Wang TianTian, Wang Xinyan
Department of Ear-Nose-Throat (ENT), Shandong Provincial Third Hospital, Cheeloo College of Medicine, Shandong University, No.11 Wuyingshan Middle Road, Tianqiao District, Jinan, 250031 Shandong China.
Department of Ear-Nose-Throat (ENT), Jinan Fourth People's Hospital, Jinan, 250031 Shandong China.
3 Biotech. 2021 Jan;11(1):25. doi: 10.1007/s13205-020-02593-8. Epub 2021 Jan 3.
This study was performed to probe the clinical significance of serum lncRNA FOXP4-AS1 in nasopharyngeal carcinoma (NPC) tumorigenesis. LncRNA FOXP4-AS1 from nasopharyngeal carcinoma patients and healthy volunteers were abstracted and converged. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of FOXP4-AS1. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of FOXP4-AS1. Kaplan-Meier survival analysis and log-rank test were used to assess the patients' survival prognosis. Independent risk factors for overall survival (OS) and progression-free survival (PFS) were assessed by univariate and multivariate cox proportional hazards regression analysis. In this study, we observed that the levels of FOXP4-AS1 were significantly upregulated in nasopharyngeal carcinoma patients compared to healthy volunteers. Besides, the expression of FOXP4-AS1 was closely associated with T stage, lymph node metastasis, and clinical stage. Meanwhile, ROC analysis found that FOXP4-AS1 had diagnostic values to distinguish tumor patients from healthy volunteers. Furthermore, patients with high FOXP4-AS1 expression level had poorer OS and PFS than those with low FOXP4-AS1 expression. Finally, univariate and multivariate Cox proportional hazards regression analysis found that the T stage, lymph node metastasis, clinical stage, FOXP4-AS1 expression might be independent risk factors for OS and PFS of nasopharyngeal carcinoma patients. This study firstly clarified that FOXP4-AS1 was overexpressed in nasopharyngeal carcinoma. And FOXP4-AS1 may act as a diagnostic and prognostic biomarker, and hopeful therapeutic target for nasopharyngeal carcinoma patients.
本研究旨在探讨血清长链非编码RNA FOXP4-AS1在鼻咽癌发生中的临床意义。提取并汇总了鼻咽癌患者和健康志愿者的长链非编码RNA FOXP4-AS1。采用定量实时聚合酶链反应（qRT-PCR）检测FOXP4-AS1的表达。采用受试者工作特征（ROC）曲线评估FOXP4-AS1的诊断价值。采用Kaplan-Meier生存分析和对数秩检验评估患者的生存预后。通过单因素和多因素Cox比例风险回归分析评估总生存（OS）和无进展生存（PFS）的独立危险因素。在本研究中，我们观察到与健康志愿者相比，鼻咽癌患者中FOXP4-AS1水平显著上调。此外，FOXP4-AS1的表达与T分期、淋巴结转移及临床分期密切相关。同时，ROC分析发现FOXP4-AS1对区分肿瘤患者和健康志愿者具有诊断价值。此外，FOXP4-AS1表达水平高的患者的OS和PFS比FOXP4-AS1表达水平低的患者差。最后，单因素和多因素Cox比例风险回归分析发现，T分期、淋巴结转移、临床分期、FOXP4-AS1表达可能是鼻咽癌患者OS和PFS的独立危险因素。本研究首次阐明FOXP4-AS1在鼻咽癌中过表达。并且FOXP4-AS1可能作为鼻咽癌患者的诊断和预后生物标志物以及有希望的治疗靶点。
