Siregar Safendra, Sasongko Noegroho Bambang, Adriansjah Ricky, Mustafa Akhmad, Wijayanti Zola
Department of Urology, Faculty of Medicine Universitas Padjadjaran, Hasan Sadikin General Hospital, Banding, Indonesia.
Res Rep Urol. 2021 Jan 6;13:1-8. doi: 10.2147/RRU.S283861. eCollection 2021.
Testicular torsion is a sudden rotation of the testis towards its axis, which causes the twisting of the spermatic cord. Post-detorsion reperfusion will cause inflammation and trigger oxidative stress, which generates reactive oxygen species (ROS). Malondialdehyde (MDA) is an organic compound formed from ROS frequently used as an oxidative stress biomarker during ischemia and ischemia-reperfusion injury. In some organs, stem cell administration on the damaged organ is essential in preventing cellular damage and death. This study aimed to learn about the effect of hADSC administration on an ischemia-reperfusion injury.
A total of 22 Wistar rats divided into 5 groups, two groups each consist of 5 male Wistar rats with testicular torsion model without hADSC therapy (group I), while 2 other groups consist of 5 rats with testicular torsion model were given 1.0×10 cells intratesticular hADSC injection 30 minutes after testicular detorsion (group II). Both groups were euthanized at 1 and 4 weeks of observation. The last group consists of 2 rats without any treatment or model (negative control group). Following euthanasia, testicular tissue was harvested for MDA expression measurement using ELISA and histopathological examination. Statistical analysis using an one way ANOVA was done with SPSS version 21.0.
The result of MDA examination using the ELISA method has shown a concentration difference between group I (control) and group II (hADSC treatment). Testicular MDA concentration in the treatment group was significantly lower on the 1st and 4th week of observation (p=0.000, p=0.016). Post hoc analysis showed no statistically different between therapy and healthy group (p=0.972). On histopathological examination, Johnsen score in the treatment group was significantly higher on the 4th week of observation (p=0.044). Post hoc analysis showed no statistically different between therapy and healthy group (p=0.195).
Intratesticular hADSC administration can inhibit ROS formation due to ischemia-reperfusion injury in testicular tissue after testicular detorsion in Wistar rats.
睾丸扭转是睾丸围绕其轴的突然旋转，导致精索扭转。扭转后再灌注会引起炎症并引发氧化应激，产生活性氧（ROS）。丙二醛（MDA）是一种由ROS形成的有机化合物，在缺血和缺血再灌注损伤期间常被用作氧化应激生物标志物。在一些器官中，向受损器官施用干细胞对于预防细胞损伤和死亡至关重要。本研究旨在了解人脂肪间充质干细胞（hADSC）施用对缺血再灌注损伤的影响。
总共22只Wistar大鼠分为5组，两组各由5只患有睾丸扭转模型且未接受hADSC治疗的雄性Wistar大鼠组成（I组），而另外两组各由5只患有睾丸扭转模型的大鼠组成，在睾丸扭转解除30分钟后给予1.0×10个细胞的睾丸内hADSC注射（II组）。两组在观察1周和4周时实施安乐死。最后一组由2只未接受任何治疗或未建立模型的大鼠组成（阴性对照组）。安乐死后，采集睾丸组织用于通过酶联免疫吸附测定（ELISA）测量MDA表达以及进行组织病理学检查。使用SPSS 21.0版本进行单因素方差分析的统计分析。
使用ELISA方法进行MDA检测的结果显示I组（对照组）和II组（hADSC治疗组）之间存在浓度差异。治疗组的睾丸MDA浓度在观察第1周和第4周时显著较低（p = 0.000，p = 0.016）。事后分析显示治疗组与健康组之间无统计学差异（p = 0.972）。在组织病理学检查中，治疗组的约翰森评分在观察第4周时显著更高（p = 0.044）。事后分析显示治疗组与健康组之间无统计学差异（p = 0.195）。
在Wistar大鼠睾丸扭转后，睾丸内施用hADSC可抑制睾丸组织因缺血再灌注损伤而产生的ROS形成。
