Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung 40227, Taiwan.
Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County 35053, Taiwan.
Int J Mol Sci. 2023 Apr 11;24(8):7062. doi: 10.3390/ijms24087062.
Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) scaffolds were prepared for human adipose-derived stem cell (hADSC) proliferation with a homemade functionally closed process bioreactor system; the feasibility of cultured hADSC spheres in heterologous stem cell therapy for OA treatment was then evaluated. hADSC spheres were collected from Ca-Ag scaffolds by removing calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation. In this study, 2D-cultured individual hADSCs or hADSC spheres were evaluated for treatment efficacy in a monosodium iodoacetate (MIA)-induced OA rat model. The results of gait analysis and histological sectioning showed that hADSC spheres were more effective at relieving arthritis degeneration. The results of serological and blood element analyses of hADSC-treated rats indicated that the hADSC spheres were a safe treatment in vivo. This study demonstrates that hADSC spheres are a promising treatment for OA and can be applied to other stem cell therapies or regenerative medical treatments.
骨关节炎（OA）是一种退行性疾病，会引起疼痛、软骨变形和关节炎症。间充质干细胞（MSCs）是治疗 OA 的潜在治疗剂。然而，MSCs 的 2D 培养可能会影响其特性和功能。在这项研究中，使用自制的功能封闭过程生物反应器系统，制备了钙-藻酸盐（Ca-Ag）支架以促进人脂肪来源的干细胞（hADSC）增殖；然后评估了在异体干细胞治疗 OA 治疗中培养的 hADSC 球体的可行性。通过 EDTA 螯合去除钙离子，从 Ca-Ag 支架中收集 hADSC 球体。在这项研究中，评估了 2D 培养的单个 hADSCs 或 hADSC 球体在单碘乙酸盐（MIA）诱导的 OA 大鼠模型中的治疗效果。步态分析和组织切片的结果表明，hADSC 球体在缓解关节炎退化方面更有效。hADSC 处理大鼠的血清学和血液元素分析结果表明，hADSC 球体在体内是一种安全的治疗方法。这项研究表明，hADSC 球体是治疗 OA 的一种有前途的方法，可应用于其他干细胞治疗或再生医学治疗。
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