Zasadzinski J A, Schneir J, Gurley J, Elings V, Hansma P K
Department of Chemical and Nuclear Engineering, University of California, Santa Barbara 93106.
Science. 1988 Feb 26;239(4843):1013-5. doi: 10.1126/science.3344420.
The high resolution of the scanning tunneling microscope (STM) makes it a potentially important tool for the study of biomaterials. Biological materials can be imaged with the STM by a procedure in which fluid, nonconductive biomaterials are replaced by rigid and highly conductive freeze-fracture replicas. The three-dimensional contours of the ripple phase of dimyristoylphosphatidylcholine bilayers were imaged with unprecedented resolution with commercial STMs and standard freeze-fracture techniques. Details of the ripple amplitude, asymmetry, and configuration unobtainable by electron microscopy or x-ray diffraction can be observed relatively easily with the STM.
扫描隧道显微镜（STM）的高分辨率使其成为研究生物材料的潜在重要工具。通过一种将流体、非导电生物材料替换为刚性且高导电的冷冻断裂复制品的程序，生物材料可用STM成像。利用商用STM和标准冷冻断裂技术，二肉豆蔻酰磷脂酰胆碱双层波纹相的三维轮廓以前所未有的分辨率成像。通过STM可以相对容易地观察到电子显微镜或X射线衍射无法获得的波纹幅度、不对称性和构型的细节。
