Melchior D L, Bruggemann E P, Steim J M
Biochim Biophys Acta. 1982 Aug 25;690(1):81-8. doi: 10.1016/0005-2736(82)90241-3.
Lipid bilayers and biomembranes produce nearly identical calorimeter scans regardless of whether they are slowly cooled under near-equilibrium conditions or rapidly frozen at rates used in freeze-fracture electron microscopy. Except for the melting of ice at 273 K, for both cooling regimens no significant thermal events occur from 100 K to the usual gel to liquid crystal transition. The gel to liquid crystal transition itself is somewhat altered by rapid cooling when bilayers contain mixed lipid species. Combined with X-ray diffraction studies, the results indicate that quickly frozen bilayers are crystalline, but that the crystalline domains are quite small or otherwise disordered. In contrast to the behavior of lipids in bilayers, hexagonal-phase calcium cardiolipin easily forms a glass upon cooling.
脂质双层和生物膜产生几乎相同的量热扫描结果，无论它们是在接近平衡的条件下缓慢冷却，还是以用于冷冻断裂电子显微镜的速率快速冷冻。除了在273K时冰的融化外，对于这两种冷却方式，从100K到通常的凝胶到液晶转变都没有明显的热事件发生。当双层含有混合脂质种类时，快速冷却会使凝胶到液晶的转变本身有所改变。结合X射线衍射研究，结果表明快速冷冻的双层是结晶的，但结晶域相当小或以其他方式无序。与双层中脂质的行为相反，六方相的心磷脂钙在冷却时很容易形成玻璃态。
