Bhaumik Jayeeta, Thakur Neeraj S, Aili Pavan K, Ghanghoriya Amit, Mittal Amit K, Banerjee Uttam C
Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar 160062, Punjab, India.
ACS Biomater Sci Eng. 2015 Jun 8;1(6):382-392. doi: 10.1021/ab500171a. Epub 2015 May 19.
Bioinspired synthesis of nanomaterials is highly advantageous as a natural and cost-effective resource. Development of noble metal nanotheranostic agents was achieved through bioinspired synthetic routes. These biosynthesized nanoparticles were characterized by various analytical techniques including absorption spectroscopy, FTIR and electron microscopy (SEM and TEM). A large number of medicinal plants were screened, among which (PF, vajradanti) and (CS, green tea) were found to produce nanomaterials with higher yields. Plant (PF and CS) mediated metallic nanoparticles had added advantage of metal reduction and simultaneous phytochemical capping over chemically synthesized procedures, which require multiple reagents. Antioxidant potential of the nanomaterials was determined by in vitro antioxidant assays confirming substantial antioxidant properties, which was due to the presence of phytochemicals on the nanoparticle surface. Flavonoids and catechins on the nanomaterial surface served as the supplier of hydroxyl groups for further derivatization. The surface of the nanoparticles was engineered by conjugating imaging and therapeutic moieties, resulting in the formation of theranostic nanoagents. The multimodal agents were characterized and the extent of drug loading was determined to validate the efficacy of those nanoconjugates. These bioinspired multimodal nanoprobes can serve as essential diagnostic and therapeutic tools in ongoing biomedical research.
受生物启发的纳米材料合成作为一种天然且具有成本效益的资源具有很大优势。通过受生物启发的合成路线实现了贵金属纳米诊疗剂的开发。这些生物合成的纳米颗粒通过各种分析技术进行表征，包括吸收光谱、傅里叶变换红外光谱以及电子显微镜（扫描电子显微镜和透射电子显微镜）。对大量药用植物进行了筛选，其中（PF，金刚齿）和（CS，绿茶）被发现能以更高产率生产纳米材料。植物（PF和CS）介导的金属纳米颗粒比化学合成方法具有额外优势，即金属还原和同时进行植物化学封端，而化学合成方法需要多种试剂。通过体外抗氧化试验确定了纳米材料的抗氧化潜力，证实其具有显著的抗氧化特性，这归因于纳米颗粒表面存在植物化学物质。纳米材料表面的黄酮类化合物和儿茶素作为进一步衍生化的羟基供应者。通过共轭成像和治疗部分对纳米颗粒表面进行工程改造，从而形成诊疗纳米剂。对这些多模态试剂进行了表征，并确定了载药量以验证那些纳米缀合物的功效。这些受生物启发的多模态纳米探针可作为当前生物医学研究中必不可少的诊断和治疗工具。
