Dada Adewumi Oluwasogo, Adekola Folahan Amoo, Dada Fehintoluwa Elizabeth, Adelani-Akande Adunola Tabitha, Bello Micheal Oluwasesan, Okonkwo Chidiogo Rita, Inyinbor Adejumoke Abosede, Oluyori Abimbola Peter, Olayanju Adeniyi, Ajanaku Kolawole Oluseyi, Adetunji Charles Oluwaseun
Industrial Chemistry Programme, Nanotechnology Laboratory, Department of Physical Sciences, (Science and Technology Research Cluster), College of Pure and Applied Sciences, Landmark University, P.M.B.1001, Omu-Aran, Kwara State, Nigeria.
Department of Industrial Chemistry, University of Ilorin, P.M.B 1515, Unilorin, Nigeria.
Heliyon. 2019 Oct 2;5(10):e02517. doi: 10.1016/j.heliyon.2019.e02517. eCollection 2019 Oct.
Single pot green synthesis of silver nanoparticles (AgNPs) was successfully carried out using medicinal plant extract of via bottom-up approach. Five imperative operational parameters (pH, contact time, concentration, volume ratio and temperature) pivotal to the synthesis of silver nanoparticles were investigated. The study showed pH 9, 90 min contact time, 0.001 M Ag concentration, volume ratio 1:9 (extract: Ag solution), and temperature between 90 - 100 °C were important for the synthesis of silver nanoparticles (AW-AgNPs). Phytochemical screening confirmed the presence of saponins, flavonoids, phenols and triterpenes for A. . These phytomolecules served as both capping and stabilizing agent in the green synthesis of silver nanoparticles. AW-AgNPs was characterized by UV-Vis Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX). The surface Plasmon resonance (SPR) was observed at 450 nm which is a characteristic absorbance region of AW-AgNPs formation as a result of the collective oscillation of free electron of silver nanoparticles. FTIR Spectroscopy confirmed the presence of functional groups responsible for bioreduction of Ag. SEM and TEM results confirmed a well dispersed AW-AgNPs of spherical shape. EDX shows the elemental distribution and confirmed AgNPs with a characteristic intense peak at 3.0 keV. AW-AgNPs showed significant inhibition against selected Gram negative and Gram positive prevailing bacteria. AW-AgNPs can therefore be recommended as potential antimicrobial and therapeutic agent against multidrug resistant pathogens.
采用自下而上的方法，成功地利用药用植物提取物单锅绿色合成了银纳米颗粒（AgNPs）。研究了对银纳米颗粒合成至关重要的五个关键操作参数（pH值、接触时间、浓度、体积比和温度）。研究表明，pH值为9、接触时间为90分钟、Ag浓度为0.001 M、体积比为1:9（提取物：Ag溶液）以及温度在90 - 100°C之间对于合成银纳米颗粒（AW-AgNPs）很重要。植物化学筛选证实了[植物名称]中存在皂苷、黄酮类化合物、酚类和三萜类化合物。这些植物分子在银纳米颗粒的绿色合成中既作为封端剂又作为稳定剂。通过紫外可见光谱、傅里叶变换红外（FTIR）光谱、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和能量色散X射线（EDX）对AW-AgNPs进行了表征。在450 nm处观察到表面等离子体共振（SPR），这是由于银纳米颗粒自由电子的集体振荡导致AW-AgNPs形成的特征吸收区域。FTIR光谱证实了负责Ag生物还原的官能团的存在。SEM和TEM结果证实了形状为球形的AW-AgNPs分散良好。EDX显示了元素分布，并确认了在3.0 keV处有特征强峰的AgNPs。AW-AgNPs对选定的革兰氏阴性和革兰氏阳性优势细菌表现出显著的抑制作用。因此，AW-AgNPs可被推荐为针对多重耐药病原体的潜在抗菌和治疗剂。
