Du Chuang, Collins Will, Cantley Will, Sood Disha, Kaplan David L
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, United States.
ACS Biomater Sci Eng. 2017 Oct 9;3(10):2235-2246. doi: 10.1021/acsbiomaterials.7b00318. Epub 2017 Aug 28.
Electrophysiology is a powerful tool to examine cellular functions, but the use of the techniques remains challenging outside of physiology and neuroscience fields. We aim to provide a practical methods guide for electrophysiological recordings to nonexperts in the field to help with the utility of these important research tools. We focus on two techniques that are critical in the context of tissue engineering, whole-cell patch clamp recording for assessing cellular functions and extracellular field potential recording for evaluating network activities. Specific examples are presented to demonstrate how the techniques were applied to tissue engineering studies.
电生理学是研究细胞功能的有力工具，但在生理学和神经科学领域之外，这些技术的应用仍然具有挑战性。我们旨在为该领域的非专业人员提供一份电生理记录的实用方法指南，以帮助他们使用这些重要的研究工具。我们重点介绍了在组织工程背景下至关重要的两种技术，即用于评估细胞功能的全细胞膜片钳记录和用于评估网络活动的细胞外场电位记录。文中给出了具体实例，以展示这些技术如何应用于组织工程研究。
