Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
International Ginseng and Herb Research Institute, 25 Insamgwangjang-ro, Geumsan-eup, Geumsan-gun 32724, Chungcheongnam-do, Korea.
Molecules. 2021 Jan 12;26(2):367. doi: 10.3390/molecules26020367.
Diabetes increases the incidence rate of chronic renal disease. Pectin-lyase-modified ginseng (GS-E3D), with enhanced ginsenoside Rd content, has been newly developed. In this study, renal protective roles of GS-E3D in type-2 diabetic db/db mice were investigated. The generation of reactive oxygen species (ROS) induced by high glucose (25 mM) was reduced by ES-E3D (75%) and ginsenoside Rd (60%). Diabetic db/db mice received 100 or 250 mg/kg/day of GS-E3D daily via oral gavage for 6 weeks. Albuminuria and urinary 8-hydroxy-2'-deoxyguanosine (8-OhdG, an oxidative stress marker) levels were increased in db/db mice and the levels recovered after GS-E3D treatment. In renal tissues, TUNEL-positive cells were decreased after GS-E3D treatment, and the increased apoptosis-related protein expressions were restored after GS-E3D treatment. Therefore, GS-E3D has a potent protective role in diabetes-induced renal dysfunction through antioxidative and antiapoptotic activities. These results may help patients to select a dietary supplement for diabetes when experiencing renal dysfunction.
糖尿病增加了慢性肾病的发病率。新开发了一种经果胶酶修饰的人参（GS-E3D），其人参皂苷 Rd 含量增加。本研究探讨了 GS-E3D 在 2 型糖尿病 db/db 小鼠中的肾脏保护作用。GS-E3D（75%）和人参皂苷 Rd（60%）可降低高葡萄糖（25 mM）诱导的活性氧（ROS）的产生。糖尿病 db/db 小鼠经口灌胃给予 100 或 250 mg/kg/天的 GS-E3D ，持续 6 周。白蛋白尿和尿 8-羟基-2'-脱氧鸟苷（8-OhdG，氧化应激标志物）水平在 db/db 小鼠中升高，经 GS-E3D 治疗后恢复。在肾脏组织中，TUNEL 阳性细胞在 GS-E3D 治疗后减少，并且 GS-E3D 治疗后恢复了增加的凋亡相关蛋白表达。因此，GS-E3D 通过抗氧化和抗凋亡作用在糖尿病引起的肾功能障碍中具有强大的保护作用。这些结果可能有助于患者在出现肾功能障碍时选择糖尿病的膳食补充剂。
