Lories Bram, Belpaire Tom E R, Yssel Anna, Ramon Herman, Steenackers Hans P
Centre of Microbial and Plant Genetics (CMPG), Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium.
Division of Mechatronics, Biostatistics, and Sensors (MeBioS), Department of Biosystems, KU Leuven, Leuven, Belgium.
Biofilm. 2020 May 29;2:100022. doi: 10.1016/j.bioflm.2020.100022. eCollection 2020 Dec.
biofilms are a common cause of contaminations in the food or feed industry. In a screening for novel compounds to combat biofilm-associated foodborne outbreaks, we identified agaric acid as a Typhimurium biofilm inhibitor that does not affect planktonic growth. Importantly, the remaining biofilm cells after preventive treatment with agaric acid were significantly more sensitive to the common disinfectant hydrogen peroxide. Screening of a GFP-promoter fusion library of biofilm related genes revealed that agaric acid downregulates the transcription of genes responsible for flagellar motility. Concurrently, swimming motility was completely abrogated in the presence of agaric acid, indicating that biofilm inhibition occurs via interference with the motility phenotype. Moreover, agaric acid also reduced biofilm formation of , and . Agaric acid thus shows potential as an anti-virulence compound that inhibits both motility and biofilm formation.
生物膜是食品或饲料行业污染的常见原因。在筛选用于对抗与生物膜相关的食源性疾病暴发的新型化合物时，我们鉴定出木耳酸是一种鼠伤寒沙门氏菌生物膜抑制剂，它不影响浮游生长。重要的是，用木耳酸进行预防性处理后剩余的生物膜细胞对常用消毒剂过氧化氢的敏感性显著更高。对生物膜相关基因的绿色荧光蛋白启动子融合文库进行筛选发现，木耳酸下调负责鞭毛运动的基因的转录。同时，在有木耳酸存在的情况下，游动性完全丧失，这表明生物膜抑制是通过干扰运动表型发生的。此外，木耳酸还减少了 、 和 的生物膜形成。因此，木耳酸显示出作为一种抑制运动性和生物膜形成的抗毒力化合物的潜力。
