Key Laboratory of Agricultural Microbiology of Guizhou Province, College of Agriculture, Guizhou University, Guiyang 550025, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Int J Mol Sci. 2023 Feb 25;24(5):4545. doi: 10.3390/ijms24054545.
is a valuable traditional Chinese medicinal plant. However, crops are affected by major diseases, such as brown rot. Previous studies have shown that brown rot is caused by and . To further understand the disease, we studied the biological and genome characteristics of these pathogenic fungi. Here, we found that the optimum growth temperature and pH of (strain QK8) and (strain SX13) were 28 °C and pH 7, and 30 °C and pH 9, respectively. An indoor virulence test showed that oxime tebuconazole, tebuconazole, and tetramycin had significant bacteriostatic effects on the two species. The genomes of QK8 and SX13 were assembled, and it was found that there was a certain gap in the size of the two fungi. The size of strain QK8 was 51,204,719 bp and that of strain SX13 was 55,171,989 bp. Afterwards, through phylogenetic analysis, it was found that strain QK8 was closely related to , while strain SX13 was closely related to . Compared with the published whole-genome data for these two strains, the genome information obtained here is more complete; the assembly and splicing reach the chromosome level. The biological characteristics and genomic information we provide here lay the foundation for further research on brown rot.
是一种有价值的中药材。然而，其作物受到多种主要病害的影响，如褐腐病。先前的研究表明，褐腐病是由 和 引起的。为了进一步了解这种疾病，我们研究了这些致病真菌的生物学和基因组特征。在这里，我们发现 （菌株 QK8）和 （菌株 SX13）的最佳生长温度和 pH 值分别为 28°C 和 pH7，以及 30°C 和 pH9。室内毒力试验表明，肟菌·戊唑醇、戊唑醇和四霉素对两种 菌株均具有显著的抑菌作用。对 QK8 和 SX13 进行基因组组装后发现，两种真菌的大小存在一定差距。菌株 QK8 的大小为 51204719bp，菌株 SX13 的大小为 55171989bp。随后，通过系统发育分析发现，菌株 QK8 与 密切相关，而菌株 SX13 则与 密切相关。与这两个 菌株已发表的全基因组数据相比，我们在这里获得的基因组信息更加完整；组装和拼接达到染色体水平。我们提供的这些生物学特性和基因组信息为进一步研究 褐腐病奠定了基础。
