Department of Thoracic Surgery, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
Department of Radiotherapy, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.
Int J Mol Med. 2021 Mar;47(3). doi: 10.3892/ijmm.2020.4836. Epub 2021 Jan 15.
Emerging studies have indicated that the dysregulation of microRNAs (miRNAs or miRs) plays a vital role in the development and metastasis of tumors. However, the role of miR‑93‑5p in esophageal carcinoma (EC) has not been extensively reported. The present study thus focused on the role of miR‑93‑5p and its downstream target in the occurrence and development of EC. Firstly, miRNA expression profiles associated with EC were accessed from the TCGA_ESCA dataset and analyzed. Subsequently, the expression patterns of miR‑93‑5p and TGFβR2 were characterized in the human esophageal cell line, Het‑1A, and the human EC cell lines, TE‑1, Eca‑109 and EC9706, by RT‑qPCR and western blot analysis. WST‑1 assay, flow cytometry, Transwell assay, wound healing assay and bioinformatics analysis were used to explore their functions in EC cells. Finally, a dual‑luciferase reporter assay was employed to determine the targeted association between miR‑93‑5p and TGFβR2. The results revealed that the expression of miR‑93‑5p was markedly higher in EC cell lines compared with that in the normal cell line. The overexpression of miR‑93‑5p facilitated cell proliferation, migration and invasion, and inhibited cell apoptosis. Additionally, TGFβR2 was identified as a functional target of miR‑93‑5p in EC cells, as judged by a series of experiments. Furthermore, it was found that the simultaneous overexpression of miR‑93‑5p and TGFβR2 almost had no effect on the biological behaviors of EC cells. On the whole, the present study demonstrates that miR‑93‑5p promotes the proliferation, migration and invasion, and inhibits the apoptosis of EC cells by targeting TGFβR2.
新兴研究表明，微 RNA（miRNA 或 miR）的失调在肿瘤的发生和转移中起着至关重要的作用。然而，miR-93-5p 在食管癌（EC）中的作用尚未得到广泛报道。因此，本研究主要关注 miR-93-5p 及其下游靶基因在 EC 发生和发展中的作用。首先，从 TCGA_ESCA 数据集获取与 EC 相关的 miRNA 表达谱，并进行分析。随后，通过 RT-qPCR 和 Western blot 分析，检测 miR-93-5p 和 TGFβR2 在人食管细胞系 Het-1A 以及人 EC 细胞系 TE-1、Eca-109 和 EC9706 中的表达模式。采用 WST-1 检测、流式细胞术、Transwell 检测、划痕愈合检测和生物信息学分析来研究它们在 EC 细胞中的功能。最后，采用双荧光素酶报告基因检测来确定 miR-93-5p 和 TGFβR2 之间的靶向关系。结果显示，miR-93-5p 在 EC 细胞系中的表达明显高于正常细胞系。过表达 miR-93-5p 促进细胞增殖、迁移和侵袭，并抑制细胞凋亡。此外，通过一系列实验证实，TGFβR2 是 EC 细胞中 miR-93-5p 的功能靶基因。此外，还发现 miR-93-5p 和 TGFβR2 的同时过表达对 EC 细胞的生物学行为几乎没有影响。总的来说，本研究表明，miR-93-5p 通过靶向 TGFβR2 促进 EC 细胞的增殖、迁移和侵袭，抑制细胞凋亡。
