Institute of Environmental Health and Ecological Security, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.
J Sci Food Agric. 2021 Aug 30;101(11):4490-4497. doi: 10.1002/jsfa.11089. Epub 2021 Feb 1.
The ultrasensitive monitoring strategy of zearalenone (ZEN) is essential and desirable for food safety and human health. In the present study, a coupling of gold nanoparticles-DNA barcode and direct competitive immunoassay-based real-time polymerase chain reaction signal amplification (RT-IPCR) for ZEN close to the sensitivity of PCR-like levels is described and evaluated.
The RT-IPCR benefited from the use of a DNA barcode and RT-PCR detection strategy, thus resulting in ultrasensitive and simple detection for ZEN. Under the optimal RT-IPCR, the linear range of detection was from 0.5 to 1000 pg mL and the limit of detection was 0.5 pg mL , which was 400-fold lower than the enzyme-linked immunosorbent assay. The detection procedure was simplified and the detection time was shortened. The specificity, accuracy and precision of the RT-IPCR confirmed a high performance. ZEN-positive contamination levels were from 0.056 to 152.12 ng g by the RT-IPCR, which was demonstrated to be highly reliable by liquid chromatography-tandem mass spectrometry.
The proposed RT-IPCR could be used as an alternative for detecting ZEN with satisfactory ultrasensitivity, simplicity, low cost and high-throughput. The present study could provide a strategy for the ultrasensitive detection of the small molecule with a simple and practical approach, which has significant appeal and application prospects.
对玉米赤霉烯酮（ZEN）进行超灵敏监测对于食品安全和人类健康至关重要。本研究描述并评估了一种金纳米粒子-DNA 条码与基于直接竞争免疫测定的实时聚合酶链反应信号放大（RT-IPCR）相结合的方法，该方法可接近 PCR 样水平的灵敏度检测 ZEN。
RT-IPCR 得益于 DNA 条码和 RT-PCR 检测策略的应用，从而实现了对 ZEN 的超灵敏和简单检测。在最佳的 RT-IPCR 条件下，检测线性范围为 0.5 至 1000 pg mL，检测限为 0.5 pg mL，比酶联免疫吸附测定低 400 倍。检测程序得到简化，检测时间缩短。RT-IPCR 的特异性、准确性和精密度证实了其高性能。通过 RT-IPCR 检测到的 ZEN 阳性污染水平为 0.056 至 152.12 ng g，这与液相色谱-串联质谱法高度可靠。
提出的 RT-IPCR 可用于检测 ZEN，具有令人满意的超灵敏度、简单、低成本和高通量的特点。本研究为小分子的超灵敏检测提供了一种简单实用的策略，具有重要的吸引力和应用前景。
