Lv Ruichan, Feng Miao, Liu Jun, Jiang Xue, Yuan Huijuan, Yan Ruyu, Tian Jie
Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710071, China.
Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
ACS Biomater Sci Eng. 2019 Sep 9;5(9):4683-4691. doi: 10.1021/acsbiomaterials.9b00688. Epub 2019 Jul 16.
In this research, a multimodal imaging platform guided photodynamic theranostics under 808 nm was designed using a NaErF:Tm@NaYF:Yb@NaLuF:Nd,Yb-ZnPc structure. Unlike conventional codoped Yb/Er system, Er ions as activator and sensitizer were used to improve the up-conversion energy transfer processes. Furthermore, higher energy transfer processes between Er ions could be obtained through doped 1% Tm ions as an energy trapping center in the NaErF. This platform could emit much brighter upconversion luminescence (UCL) (124-fold enhancement for red emission) and short wavelength infrared (SWIR) emission under single 808 nm laser excitation. Importantly, the SWIR imaging with higher resolution and better signal-to-noise ratio can pass the blood-brain barrier to see the brain vessels. Because of the enhanced red emission, the UCL nanoparticles were combined with ZnPc agent to exhibit photodynamic therapy (PDT) effect, and its distribution and excretion could be detected by the photoacoustic (PA) imaging under single near-infrared (NIR) laser. Thus, this platform could be used as multimodal imaging (SWIR, PA, CT, and UCL) guided PDT agent under single 808 nm laser.
在本研究中，使用NaErF:Tm@NaYF:Yb@NaLuF:Nd,Yb-ZnPc结构设计了一种808 nm下的多模态成像平台引导的光动力诊疗方法。与传统的共掺杂Yb/Er系统不同，将Er离子用作激活剂和敏化剂以改善上转换能量转移过程。此外，通过在NaErF中掺杂1%的Tm离子作为能量俘获中心，可以获得Er离子之间更高的能量转移过程。该平台在单808 nm激光激发下可发出更亮的上转换发光（UCL）（红色发射增强124倍）和短波长红外（SWIR）发射。重要的是，具有更高分辨率和更好信噪比的SWIR成像可以穿过血脑屏障以观察脑血管。由于红色发射增强，UCL纳米颗粒与ZnPc试剂结合表现出光动力疗法（PDT）效果，并且其分布和排泄可以通过单近红外（NIR）激光下的光声（PA）成像进行检测。因此，该平台可作为单808 nm激光下的多模态成像（SWIR、PA、CT和UCL）引导的PDT试剂。
