利用潜在的即时检测电化学免疫传感器快速诊断 SARS-CoV-2:展望未来。

Rapid diagnosis of SARS-CoV-2 using potential point-of-care electrochemical immunosensor: Toward the future prospects.

机构信息

Microfluidics and MEMS Centre, CSIR - Advanced Materials and Processes Research Institute (AMPRI), Bhopal, India.

Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.

出版信息

Int Rev Immunol. 2021;40(1-2):126-142. doi: 10.1080/08830185.2021.1872566. Epub 2021 Jan 15.

Abstract

Coronavirus disease (COVID-19) is an emerging and highly infectious disease making global public health concern and socio-economic burden. It is caused due to Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2). It has the tendency to spread rapidly through person-to-person. Currently, several molecular diagnostic platforms such as PCR, qRT-PCR, reverse transcription loop-mediated isothermal amplification (RT-LAMP), CRISPR are utilized for the diagnosis of SARS-CoV-2. These conventional techniques are costly, time consuming and require sophisticated instrumentation facility with well trained personnel for testing. Hence, it is tough to provide testing en-masse to the people in developing countries. On the other hand, several serological biosensors such as lateral flow immunosensor, optical, electrochemical, microfluidics integrated electrochemical/fluorescence is currently utilized for the diagnosis of SARS-CoV-2. In current pandemic situation, there is an urgent need of rapid and efficient diagnosis on mass scale of SARS-CoV-2 for early stage detection. Early monitoring of viral infections can help to control and prevent the spreading of infections in large chunk of population. In this review, the SARS-CoV-2 and their biomarkers in biological samples, collection of samples and recently reported potential electrochemical immunosensors for the rapid diagnosis of SARS-CoV-2 are discussed.

摘要

冠状病毒病(COVID-19)是一种新兴的高传染性疾病,引起了全球公共卫生关注和社会经济负担。它是由严重急性呼吸系统综合症冠状病毒(SARS-CoV-2)引起的。它有通过人与人之间迅速传播的趋势。目前,有几种分子诊断平台,如 PCR、qRT-PCR、逆转录环介导等温扩增(RT-LAMP)、CRISPR,用于 SARS-CoV-2 的诊断。这些传统技术成本高、耗时且需要复杂的仪器设备和经过良好培训的人员进行测试。因此,很难在发展中国家大规模地为人们提供测试。另一方面,几种血清学生物传感器,如侧向流动免疫传感器、光学、电化学、微流控集成电化学/荧光传感器,目前用于 SARS-CoV-2 的诊断。在当前的大流行情况下,迫切需要大规模快速有效地诊断 SARS-CoV-2,以便进行早期检测。早期监测病毒感染有助于控制和预防大量人群的感染传播。在这篇综述中,讨论了 SARS-CoV-2 及其在生物样本中的生物标志物、样本采集以及最近报道的用于 SARS-CoV-2 快速诊断的潜在电化学免疫传感器。

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