Horticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74 Xuefu Road, Harbin, 150000, Heilongjiang, China.
Mol Genet Genomics. 2021 Mar;296(2):379-390. doi: 10.1007/s00438-020-01751-0. Epub 2021 Jan 15.
Common bean (Phaseolus vulgaris L.) is a short-day plant and its flowering time, and consequently, pod yield and quality is influenced by photoperiod. In this study, the photoperiodic-sensitive variety 'Hong jin gou', which flowers 31 days (d) earlier in short-day than in long-day, was used as the experimental material. Samples were collected to determine the growth and photosynthetic parameters in each daylength treatment, and transcriptome and metabolome data were conducted. We identified eight genes related to flowering by further screening for differentially expressed genes. These genes function to regulate the biological clock. The combination of differentially expressed genes and metabolites, together with the known regulation network of flowering time and the day-night expression pattern of related genes allow us to speculate on the regulation of flowering time in the common bean and conclude that TIMING OF CAB EXPRESSION1 (TOC1) plays a pivotal role in the network and its upregulation or downregulation causes corresponding changes in the expression of downstream genes. The regulatory network is also influenced by gibberellic acid (GA) and jasmonic acid (JA). These regulatory pathways jointly comprise the flowering regulatory network in common bean.
普通菜豆（Phaseolus vulgaris L.）是一种短日照植物，其开花时间，进而荚果产量和品质受光周期的影响。本研究以开花时间比长日照提前 31 天的短日照敏感品种“红豆沟”为实验材料。收集样品以确定在每种日照长度处理下的生长和光合参数，并进行转录组和代谢组数据分析。我们通过进一步筛选差异表达基因，鉴定出与开花相关的 8 个基因。这些基因的功能是调节生物钟。差异表达基因和代谢物的组合，以及已知的开花时间调控网络和相关基因的日夜表达模式，使我们能够推测普通菜豆开花时间的调控机制，并得出结论：TOC1 在网络中起关键作用，其上调或下调会导致下游基因表达的相应变化。该调控网络还受赤霉素（GA）和茉莉酸（JA）的影响。这些调控途径共同构成了普通菜豆的开花调控网络。
