Microbial Communication, Institute of Microbiology, Friedrich Schiller University Jena, Jena, Germany.
Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Jena, Germany.
PLoS One. 2021 Jan 15;16(1):e0245623. doi: 10.1371/journal.pone.0245623. eCollection 2021.
Wood is a habitat for a variety of organisms, including saprophytic fungi and bacteria, playing an important role in wood decomposition. Wood inhabiting fungi release a diversity of volatiles used as signaling compounds to attract or repel other organisms. Here, we show that volatiles of Schizophyllum commune are active against wood-decay fungi and bacteria found in its mycosphere. We identified sesquiterpenes as the biologically active compounds, that inhibit fungal growth and modify bacterial motility. The low number of cultivable wood inhabiting bacteria prompted us to analyze the microbial community in the mycosphere of S. commune using a culture-independent approach. Most bacteria belong to Actinobacteria and Proteobacteria, including Pseudomonadaceae, Sphingomonadaceae, Erwiniaceae, Yersiniaceae and Mariprofundacea as the dominating families. In the fungal community, the phyla of ascomycetes and basidiomycetes were well represented. We propose that fungal volatiles might have an important function in the wood mycosphere and could meditate interactions between microorganisms across domains and within the fungal kingdom.
木材是多种生物的栖息地，包括腐生真菌和细菌，在木材分解中起着重要作用。木材栖息真菌释放出多种挥发物，用作信号化合物，以吸引或排斥其他生物。在这里，我们表明，裂褶菌的挥发物对其菌根中的木材腐朽真菌和细菌具有活性。我们确定了倍半萜类化合物是具有生物活性的化合物，它们抑制真菌生长并改变细菌的运动性。可培养的木材栖息细菌数量较少，促使我们使用非培养方法分析裂褶菌菌根中的微生物群落。大多数细菌属于放线菌门和变形菌门，包括假单胞菌科、鞘氨醇单胞菌科、欧文氏菌科、耶尔森氏菌科和深海菌科，它们是主要的科。在真菌群落中，子囊菌门和担子菌门的真菌种类丰富。我们提出，真菌挥发物可能在木材菌根中具有重要功能，并可以介导不同领域和真菌界内微生物之间的相互作用。
