Friedrich Schiller University, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743 Jena, Germany.
Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Adolf-Reichwein-Straße 23, 07745 Jena, Germany.
Fungal Genet Biol. 2018 Mar;112:2-11. doi: 10.1016/j.fgb.2016.08.007. Epub 2016 Sep 1.
Mushrooms, such as Schizophyllum commune, have a specific odor. Whether this is linked to mating, prerequisite for mushroom formation, or also found in monokaryotic, unmated strains, was investigated with a comprehensive study on the transcriptome and proteome of this model organism. Mating interactions were investigated using a complete, cytosolic proteome map for unmated, monokaryotic, as well as for mated, dikaryotic mycelia. The regulations of the proteome were compared to transcriptional changes upon mating and to changes in smell by volatilome studies. We could show a good overlap between proteome and transcriptome data, but extensive posttranslational regulation was identified for more than 80% of transcripts. This suggests down-stream regulation upon interaction of mating partners and formation of the dikaryon that is competent to form fruiting bodies. The volatilome was shown to respond to mating by a broader spectrum of volatiles and increased emission of the mushroom smell molecules 3-octanone and 1-octen-3-ol, as well as ethanol and β-bisabolol in the dikaryon. Putatively involved biosynthetic proteins like alcohol dehydrogenases, Ppo-like oxygenases, or sesquiterpene synthases showed correlating transcriptional regulation depending on either mono- or dikaryotic stages.
蘑菇，如裂褶菌，具有特殊的气味。这种气味是否与交配有关，交配是蘑菇形成的前提，或者在单核、未交配的菌株中也存在，我们用对该模式生物的转录组和蛋白质组进行的综合研究来研究。通过对未交配的单核、单核以及交配的双核菌丝体的完整细胞质蛋白质组图谱的研究，研究了交配相互作用。将蛋白质组的调控与交配时的转录变化和挥发物研究中的气味变化进行了比较。我们可以看到蛋白质组和转录组数据之间有很好的重叠，但超过 80%的转录本都存在广泛的翻译后调控。这表明在交配伙伴相互作用和双核形成过程中有下游调控，双核形成有能力形成子实体。挥发物研究表明，挥发物谱对交配有反应，并增加了蘑菇气味分子 3-辛酮和 1-辛烯-3-醇以及双核中的乙醇和 β-双醇的排放。根据单核或双核阶段的不同，可能参与生物合成的蛋白质，如醇脱氢酶、Ppo 样加氧酶或倍半萜合酶，显示出相应的转录调控。
