School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami, Kochi 782-8502, Japan.
School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami, Kochi 782-8502, Japan.
Bioresour Technol. 2015 Dec;197:37-41. doi: 10.1016/j.biortech.2015.08.031. Epub 2015 Aug 18.
In the present study, ethanol production from polysaccharides or wood chips was conducted in a single reactor under anaerobic conditions using the white rot fungus Schizophyllum commune NBRC 4928, which produces enzymes that degrade lignin, cellulose and hemicellulose. The ethanol yields produced from glucose and xylose were 80.5%, and 52.5%, respectively. The absolute yields of ethanol per microcrystalline cellulose (MCC), xylan and arabinogalactan were 0.26g/g-MCC, 0.0419g/g-xylan and 0.0508g/g-arabinogalactan, respectively. By comparing the actual ethanol yields from polysaccharides with monosaccharide fermentation, it was shown that the rate of saccharification was slower than that in fermentation. S. commune NBRC 4928 is concluded to be suitable for CBP because it can produce ethanol from various types of sugar. From the autoclaved cedar chip, only little ethanol was produced by S. commune NBRC 4928 alone but ethanol production was enhanced by combined use of ethanol fermenting and lignin degrading fungi.
在本研究中，使用产木质素降解酶的白腐菌糙皮侧耳 NBRC 4928，在厌氧条件下于单反应器中从多糖或木屑生产乙醇。从葡萄糖和木糖生产的乙醇得率分别为 80.5%和 52.5%。微晶纤维素（MCC）、木聚糖和阿拉伯半乳聚糖的绝对乙醇产率分别为 0.26g/g-MCC、0.0419g/g-xylan 和 0.0508g/g-arabinogalactan。通过将多糖的实际乙醇产率与单糖发酵进行比较，表明糖化速度比发酵速度慢。糙皮侧耳 NBRC 4928 适合 CBP，因为它可以从各种类型的糖生产乙醇。从蒸煮后的雪松木屑中，单独使用糙皮侧耳 NBRC 4928 仅产生少量乙醇，但通过组合使用乙醇发酵和木质素降解真菌可提高乙醇产量。
