一种用于从严重污染材料中分离李斯特菌属的新型选择性培养基。
A new selective medium for isolating Listeria spp. from heavily contaminated material.
作者信息
Bannerman E S, Bille J
机构信息
Institute of Microbiology, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, Switzerland.
出版信息
Appl Environ Microbiol. 1988 Jan;54(1):165-7. doi: 10.1128/aem.54.1.165-167.1988.
Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride agar, the acriflavine-ceftazidime agar recovered more Listeria isolates (98 versus 65%, P less than 0.001) more rapidly (57% after 48 h of incubation of the enrichment broth versus 35%, P less than 0.01) and in greater amounts. Acriflavine-ceftazidime selective agar medium proved to be a highly sensitive medium to recover Listeria spp. from heavily contaminated food products.
与食物相关的人类李斯特菌病暴发凸显了筛查食物中是否存在李斯特菌分离株的重要性和必要性。为此开发了一种将吖啶黄（10毫克/升）与头孢他啶（50毫克/升）结合的分离株选择性琼脂培养基。共培养了1099份奶酪生产样本，从中生长出157株李斯特菌分离株（14.3%）。与改良麦克布莱德琼脂相比，吖啶黄-头孢他啶琼脂能更快地检出更多的李斯特菌分离株（98%对65%，P<0.001），培养48小时后富集肉汤中生长的菌株数量更多（57%对35%，P<0.01）。吖啶黄-头孢他啶选择性琼脂培养基被证明是一种从严重污染的食品中回收李斯特菌属的高灵敏度培养基。
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