用于定量回收食源单核细胞增生李斯特菌的选择性平板培养基。
Selective plating medium for quantitative recovery of food-borne Listeria monocytogenes.
作者信息
Lachica R V
机构信息
U.S. Army Natick Research, Development and Engineering Center, Massachusetts 01760.
出版信息
Appl Environ Microbiol. 1990 Jan;56(1):167-9. doi: 10.1128/aem.56.1.167-169.1990.
Abstract
A new plating medium (lithium chloride-ceftazidime agar [LCA]) was designed to quantitatively recover food-borne Listeria monocytogenes in the form of large colonies while inhibiting most other food-borne microorganisms. This medium included brain heart infusion agar as the nutritive agar base and a combination of selective agents (LiCl, glycine anhydride, and ceftazidime). Comparison of LCA and lithium chloride-phenylethanol-moxalactam agar (LPM) indicated that both were equally effective for the enumeration of the cold-tolerant pathogen in artificially and naturally contaminated foods. However, LCA was more effective than LPM in the recovery of sublethally heat-injured cells. Moreover, Listeria colonies on LCA exhibited a more distinct bluish hue than those on LPM when viewed by the Henry oblique transillumination technique.
摘要
一种新的平板培养基（氯化锂-头孢他啶琼脂 [LCA]）被设计用于以大菌落形式定量回收食源性单核细胞增生李斯特菌，同时抑制大多数其他食源微生物。该培养基包含脑心浸液琼脂作为营养琼脂基础以及多种选择性试剂（LiCl、甘氨酸酐和头孢他啶）的组合。LCA与氯化锂-苯乙醇-莫拉卡坦琼脂（LPM）的比较表明，二者在人工和天然污染食品中对这种耐寒病原体的计数效果相同。然而，LCA在亚致死热损伤细胞的回收方面比LPM更有效。此外，当通过亨利斜射照明技术观察时，LCA上的李斯特菌菌落比LPM上的菌落呈现出更明显的蓝色调。
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