Grabowska Karolina, Żmudzki Paweł, Wróbel-Biedrawa Dagmara, Podolak Irma
Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmacognosy, Kraków, Poland.
Jagiellonian University Medical College, Faculty of Pharmacy, Department of Medicinal Chemistry, Kraków, Poland.
Planta Med. 2021 Apr;87(4):305-313. doi: 10.1055/a-1345-9377. Epub 2021 Jan 15.
The content of ursolic acid and oleanolic acid was determined in different plant parts of two species, and . To achieve optimal extraction conditions of ursolic acid and oleanolic acid from plant material, several methods including maceration, heat reflux, Soxhlet, and ultrasonic extraction, as well as various solvents (methanol, dichloromethane, ethyl acetate), were investigated and compared.For the simultaneous quantification of pentacyclic triterpenes in extracts from sp., an UPLC-MS/MS was developed and validated. The method exhibited good linearity, precision, and recovery, and it also was simple, specific, and fast. We developed the method for future application in the quality control of plant materials and botanical extracts containing ursolic acid and oleanolic acid. With regard to the triterpene constituents, both and can be used equally, and the aboveground parts of both species, but the leaves especially, are abundant sources of ursolic acid (7.1 - 7.5 mg/g dry weight [DW]). Dichloromethane as an extractant provided the best extraction efficiency as well as selectivity to obtain extracts rich in triterpenes as compared to methanol and ethyl acetate, regardless of the particular extraction technique. Dry dichloromethane extracts from aerial parts of . obtained by the heat reflux method resulted in products with a high content of UA (17 - 25% w/w) are considered to be convenient and rich sources of this compound.
测定了两个物种不同植物部位中熊果酸和齐墩果酸的含量。为了从植物材料中获得熊果酸和齐墩果酸的最佳提取条件，研究并比较了浸渍法、热回流法、索氏提取法和超声提取法等几种方法，以及各种溶剂（甲醇、二氯甲烷、乙酸乙酯）。为了同时定量测定某物种提取物中的五环三萜，开发并验证了一种超高效液相色谱-串联质谱法（UPLC-MS/MS）。该方法具有良好的线性、精密度和回收率，且简单、特异、快速。我们开发该方法以便将来应用于含熊果酸和齐墩果酸的植物材料和植物提取物的质量控制。关于三萜成分，两个物种均可同等使用，且两个物种的地上部分，尤其是叶子，是熊果酸的丰富来源（7.1 - 7.5毫克/克干重[DW]）。与甲醇和乙酸乙酯相比，无论采用何种具体提取技术，二氯甲烷作为萃取剂在获得富含三萜的提取物方面均具有最佳的提取效率和选择性。通过热回流法从某物种地上部分获得的干燥二氯甲烷提取物所得到的产品中熊果酸含量较高（17 - 25% w/w），被认为是该化合物方便且丰富的来源。
