Grabowska Karolina, Amanowicz Kinga, Paśko Paweł, Podolak Irma, Galanty Agnieszka
Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9, 30-688 Kraków, Poland.
Department of Food Chemistry and Nutrition, Faculty of Pharmacy, Jagiellonian University, Medical College, Medyczna 9, 30-688 Kraków, Poland.
Plants (Basel). 2022 Aug 26;11(17):2217. doi: 10.3390/plants11172217.
The dried L. herb has a long history of use in traditional medicine. Its therapeutic potential is related to the presence of phenolic compounds. To optimize extraction efficiency the effect of the use of different techniques (HRE-heat reflux extraction, I/ME-infusion combined with maceration, UE-sonication and SE-Soxhlet extraction), various solvents (water and ethanol) and processing time (15 min to 2 h) on phenolics content was investigated. The HPLC method was applied to determine and compare the content of phenolic acids (rosmarinic, chlorogenic, protocatechuic) and flavonoids (rutin, isoquercetin) in the extracts. Furthermore, the cytotoxic activity of the extracts was examined for the first time against human cancer and normal cells of skin origin (A375, HTB140, HaCaT) and gastrointestinal origin (Caco-2 and HT-29, HepG2). In addition, the antioxidant potential was evaluated using the DPPH and FRAP method. The I/ME-water and HRE/ethanol procedures turned out to be optimal for obtaining extracts of dried L. herb rich in bioactive phenolics. These extracts exhibited high antioxidant activity, correlated with the content of the compounds analyzed. Furthermore, the extracts of the dried herb were not toxic to normal human cells, indicating its safe use both internally and externally.
干燥的[草药名称]在传统医学中有悠久的使用历史。其治疗潜力与酚类化合物的存在有关。为了优化提取效率，研究了不同技术（HRE - 热回流提取、I/ME - 浸渍联合渗漉、UE - 超声处理和SE - 索氏提取）、各种溶剂（水和乙醇）以及处理时间（15分钟至2小时）对酚类含量的影响。采用高效液相色谱法测定并比较提取物中酚酸（迷迭香酸、绿原酸、原儿茶酸）和黄酮类化合物（芦丁、异槲皮苷）的含量。此外，首次检测了提取物对源自皮肤的人类癌细胞和正常细胞（A375、HTB140、HaCaT）以及源自胃肠道的细胞（Caco - 2、HT - 29、HepG2）的细胞毒性活性。另外，使用DPPH和FRAP方法评估了抗氧化潜力。结果表明，I/ME - 水提取法和HRE/乙醇提取法是获得富含生物活性酚类的干燥[草药名称]提取物的最佳方法。这些提取物表现出高抗氧化活性，与所分析化合物的含量相关。此外，干燥[草药名称]的提取物对正常人类细胞无毒，表明其在体内和体外使用均安全。
