Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter, Vienna, Austria.
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 1030, Vienna, Austria.
Nat Commun. 2021 Jan 15;12(1):387. doi: 10.1038/s41467-020-20728-6.
Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Their precise sequence information and organization remain uncharacterized. Here, using a combination of long- and short-read sequencing technologies we assemble contigs of the Arabidopsis NOR2 rDNA domain. We identify several expressed rRNA gene variants which are integrated into translating ribosomes in a tissue-specific manner. These findings support the concept of tissue specific ribosome subpopulations that differ in their rRNA composition and provide insights into the higher order organization of NOR2.
尽管生物体之间存在巨大差异，但它们的基因组仍具有一些保守特征，例如核仁组织区 (NOR)。NOR 由多个高度重复的 rDNA 基因组成，这些基因编码的催化核糖体核心 RNA 是从 45S rDNA 单位转录而来的。它们的精确序列信息和组织仍未被描述。在这里，我们使用长读和短读测序技术相结合，组装了拟南芥 NOR2 rDNA 结构域的连续序列。我们鉴定了几种表达的 rRNA 基因变体，这些变体以组织特异性的方式整合到翻译核糖体中。这些发现支持了组织特异性核糖体亚群的概念，这些亚群在 rRNA 组成上存在差异，并为 NOR2 的高级组织提供了见解。
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