Department of Medical Microbiology, Kocaeli University Faculty of Medicine, 41380, Kocaeli, Turkey.
Sci Rep. 2021 Jan 15;11(1):1608. doi: 10.1038/s41598-021-81171-1.
The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.
结直肠癌是一个严重的健康问题。该疾病的诊断主要依赖于一种有创性的程序。一种非侵入性的诊断测试，如免疫测定，可能有助于结直肠癌的诊断。本研究旨在评估抗具核梭杆菌抗体在结直肠癌（CRC）诊断中的应用。共有 78 例患者分为三组纳入本研究。使用定量聚合酶链反应检测组织中的具核梭杆菌。使用酶联免疫吸附试验检测具核梭杆菌 IgA 和 IgG。在 CRC 和癌前良性结肠疾病（P-BCD）病例中分别检测到 86.7%和 73.1%的具核梭杆菌。与健康个体相比，CRC 组的具核梭杆菌 IgA 和 IgG ELISA 试验的 OD 值更高。根据所选的截断值，IgA ELISA 试验的灵敏度在 31.8%至 95.5%之间变化。组织中具核梭杆菌含量高的患者的抗体阳性率明显高于阴性组。CRC 中的具核梭杆菌 IgA 和 IgG 抗体高于健康对照组，但 ELISA 试验的鉴别能力不足，不能作为诊断工具。
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