Immunology & Vaccinology Group. Centro de Investigaciones Biológicas Del Noroeste, S.C. (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz, B.C.S, 23090, Mexico.
Immunology & Vaccinology Group. Centro de Investigaciones Biológicas Del Noroeste, S.C. (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz, B.C.S, 23090, Mexico.
Microb Pathog. 2021 Jan;150:104735. doi: 10.1016/j.micpath.2021.104735. Epub 2021 Jan 13.
Immunostimulant and protective effects of Yarrowia lipolytica glucans against important pathogens, such as Escherichia coli, have not been investigated in goats and other ruminants. This study aimed to characterize Y. lipolytica N6-glucan (Yl-glucan) and its possible role in immunological signaling pathway activation and immunoprotection against E. coli in goat leukocytes. Characterization analyses showed that Y. lipolytica content had a mix of β and α-D-glucans, molecular weight of 3301.53 kDa and low solubility after the heat treatment. The stimulation of goat leukocytes with Yl-glucan induced protection against E. coli challenge. Remarkably, Yl-glucan and E. coli interaction increased gene expression of dectin-1 and TLR-2 receptors, signaling pathway Syk/NFκB, and cytokines, such as TNF-α and IL-10. As a consequence of signaling activation, phagocytosis, and nitric oxide production enhanced killing of pathogens. Altogether, Y. lipolytica-glucan demonstrated to possess an immunoprotective potential against E. coli through innate immune response modulation in goat leukocytes.
脂肪圆酵母葡聚糖的免疫刺激和保护作用，如大肠杆菌，尚未在山羊和其他反刍动物中进行研究。本研究旨在表征脂肪圆酵母 N6-葡聚糖（Yl-葡聚糖）及其在山羊白细胞中激活免疫信号通路和抗大肠杆菌免疫保护中的可能作用。特征分析表明，脂肪圆酵母的含量有β和α-D-葡聚糖的混合物，分子量为 3301.53 kDa，经过热处理后溶解度较低。用 Yl-葡聚糖刺激山羊白细胞可诱导对大肠杆菌的保护作用。值得注意的是，Yl-葡聚糖和大肠杆菌的相互作用增加了 dectin-1 和 TLR-2 受体、信号通路 Syk/NFκB 和细胞因子（如 TNF-α 和 IL-10）的基因表达。作为信号激活的结果，吞噬作用和一氧化氮的产生增强了病原体的杀伤。总之，脂肪圆酵母葡聚糖通过调节山羊白细胞的固有免疫反应，显示出对大肠杆菌具有免疫保护潜力。
