Instituto de Antropología de Córdoba (IDACOR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Córdoba (UNC), Córdoba, Argentina.
Centro de Investigación en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (UNC), Córdoba, Argentina.
Int J Paleopathol. 2021 Mar;32:74-79. doi: 10.1016/j.ijpp.2020.12.004. Epub 2021 Jan 13.
Detecting traces of ancient DNA of Vibrio cholerae to provide genetic information associated with the fifth cholera pandemic.
Sediment samples from the sacral foramina of four individuals were analyzed, recovered from a mass grave near an institution dedicated exclusively to the isolation and treatment of citizens infected with cholera in the late 19th century in the city of Cordoba, Argentina.
Paleogenetic techniques (ancient DNA extraction, PCR amplification, and Sanger sequencing) were applied. Specific primers for Vibrio cholerae (VCR, ctxA, ctxB, and tcpA) were designed.
By amplifying and sequencing the Vibrio cholerae repeats fragment, the infection in at least one individual was confirmed.
The synthesis of the paleogenetic results with the archaeological and historical evidence strongly supports that at least one individual from the mass grave in Cordoba, Argentina, was a victim of the fifth cholera pandemic.
Confirming the presence of the disease through multiple lines of evidence, including genetic, archaeological, and historical analyses, strengthens and affirms our understanding of the presence, effects, and potential evolutionary paths of the disease in the past.
Vibrio cholerae repeats were sequenced in one individual, while the remaining genes could not be amplified, which is likely related to gene copy number.
Paleogenetic examination of ancient samples from different locations will broaden our understanding of the origin, evolution, and past dissemination of Vibrio cholerae epidemic strains.
检测霍乱弧菌的古代 DNA 痕迹，提供与第五次霍乱大流行相关的遗传信息。
分析了来自阿根廷科尔多瓦市一家专门用于隔离和治疗 19 世纪末感染霍乱公民的机构附近一个万人坑中 4 个人的骶骨孔的沉积物样本。
应用古遗传学技术（古代 DNA 提取、PCR 扩增和 Sanger 测序）。设计了霍乱弧菌（VCR、ctxA、ctxB 和 tcpA）的特异性引物。
通过扩增和测序霍乱弧菌重复片段，证实至少有一个个体感染了霍乱弧菌。
古遗传学结果与考古学和历史学证据的综合强烈支持，阿根廷科尔多瓦万人坑中的至少一个个体是第五次霍乱大流行的受害者。
通过遗传、考古和历史分析等多种证据证实疾病的存在，加强并证实了我们对过去疾病的存在、影响和潜在进化途径的理解。
在一个个体中测序了霍乱弧菌重复序列，而其余基因无法扩增，这可能与基因拷贝数有关。
对来自不同地点的古代样本进行古遗传学检查，将拓宽我们对霍乱弧菌流行株起源、进化和过去传播的理解。
