Department of Botany, Government College University Faisalabad, Pakistan.; Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Pakistan.
Department of Botany, Government College University Faisalabad, Pakistan..
Ecotoxicol Environ Saf. 2021 Mar 15;211:111901. doi: 10.1016/j.ecoenv.2021.111901. Epub 2021 Jan 13.
The effects of fullerenol nanopriming (0, 10, 40, 80 and 120 nM concentration) on salt stressed-wheat (0 and 150 mM NaCl) were investigated under natural conditions. Salinity resulted in a shift in wheat growth pattern in the form of LAR (+ 40.9% increase) and RGR (+ 13.4% increase) while decreased NAR (- 31.7%). It also disturbed shoot and root biomass, ion uptake and reduced chlorophyll contents. Despite increase in enzyme activities, higher ROS generation (+ 48.1% O anion; and + 62.2% HO) and lipid peroxidation (+ 40.8% MDA) were detected in salt-stressed wheat plants. Possibly, the increases in enzyme activities were not up to the level to completely counteract the salinity induced oxidative stress. Nanopriming with fullerenol improved NAR (+ 8.77% to 23.2%), ROS metabolism and decreased indicators of oxidative stress. Hydropriming treatment also promoted NAR recovery by 21.9% than control plants. Compared to Na ions, improvements in shoot relative concentrations of K, Ca and P also recorded along with soluble sugars and amino acids, which improved osmotic balance. These biochemical modifications contributed to improvements in grain yield attributes (+11.8% to 18.3% in 100 grain-weight) than salinity stressed control. Hydropriming also contributed to a recovery in grain yield attributes by 12.6%. Above all, the harvested seeds from fullerenol treated plants also showed better germination and seedlings growth traits. Conclusively, we report non-toxic, growth-promoting effects of fullerenol nanoparticles on wheat crop and as a way forward; we suggest its exogenous application to recover crop productivity under saline environments.
在自然条件下，研究了富勒醇纳米引发（0、10、40、80 和 120 nM 浓度）对盐胁迫小麦（0 和 150 mM NaCl）的影响。盐度导致小麦生长模式发生变化，表现为 LAR（增加 40.9%）和 RGR（增加 13.4%），而 NAR 减少（减少 31.7%）。它还干扰了地上部和根生物量、离子吸收和减少叶绿素含量。尽管酶活性增加，但在盐胁迫小麦植株中检测到更高的 ROS 生成（O 阴离子增加 48.1%；HO 增加 62.2%）和脂质过氧化（MDA 增加 40.8%）。可能是由于酶活性的增加不足以完全抵消盐诱导的氧化应激。富勒醇纳米引发提高了 NAR（增加 8.77%至 23.2%）、ROS 代谢并降低了氧化应激指标。水引发处理也比对照植物提高了 NAR 恢复率 21.9%。与 Na 离子相比，还记录了地上部相对 K、Ca 和 P 浓度的提高，以及可溶性糖和氨基酸的提高，从而改善了渗透平衡。这些生化修饰有助于提高粒重（100 粒重增加 11.8%至 18.3%）等谷物产量性状，优于盐胁迫对照。水引发处理也有助于提高谷物产量性状 12.6%。最重要的是，从富勒醇处理过的植物中收获的种子也表现出更好的发芽和幼苗生长特性。总之，我们报告了富勒醇纳米粒子对小麦作物的无毒、促进生长的作用，作为一种前进的方式；我们建议将其外源性应用于恢复盐环境下的作物生产力。
