School of Chemistry, University College Cork, Cork, Ireland.
Tyndall National Institute, Cork, Ireland.
Electrophoresis. 2022 Oct;43(20):1944-1952. doi: 10.1002/elps.202200116. Epub 2022 Aug 27.
A simple, rapid method using CE and microchip electrophoresis with C D has been developed for the separation of four nonsteroidal anti-inflammatory drugs (NSAIDs) in the environmental sample. The investigated compounds were ibuprofen (IB), ketoprofen (KET), acetylsalicylic acid (ASA), and diclofenac sodium (DIC). In the present study, we applied for the first time microchip electrophoresis with C D detection to the separation and detection of ASA, IB, DIC, and KET in the wastewater matrix. Under optimum conditions, the four NSAIDs compounds could be well separated in less than 1 min in a BGE composed of 20 mM His/15 mM Tris, pH 8.6, 2 mM hydroxypropyl-beta-cyclodextrin, and 10% methanol (v/v) at a separation voltage of 1000-1200 V. The proposed method showed excellent repeatability, good sensitivity (LODs ranging between 0.156 and 0.6 mg/L), low cost, high sample throughputs, portable instrumentation for mobile deployment, and extremely lower reagent and sample consumption. The developed method was applied to the analysis of pharmaceuticals in wastewater samples with satisfactory recoveries ranging from 62.5% to 118%.
已经开发出一种使用 CE 和带有 C D 的微芯片电泳的简单、快速方法，用于分离环境样品中的四种非甾体抗炎药 (NSAIDs)。所研究的化合物为布洛芬 (IB)、酮洛芬 (KET)、乙酰水杨酸 (ASA) 和双氯芬酸钠 (DIC)。在本研究中，我们首次将带有 C D 检测的微芯片电泳应用于废水中 ASA、IB、DIC 和 KET 的分离和检测。在最佳条件下，四种 NSAIDs 化合物在由 20 mM His/15 mM Tris、pH 8.6、2 mM 羟丙基-β-环糊精和 10%甲醇 (v/v) 组成的 BGE 中，在 1000-1200 V 的分离电压下，可在不到 1 分钟的时间内得到很好的分离。所提出的方法具有出色的重现性、良好的灵敏度 (LOD 范围在 0.156 至 0.6 mg/L 之间)、低成本、高样品通量、可用于移动部署的便携式仪器以及极低的试剂和样品消耗。该方法已应用于废水样品中药物的分析，回收率在 62.5%至 118%之间。
