Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand.
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, 10400, Thailand.
Anal Chim Acta. 2021 Feb 1;1144:102-110. doi: 10.1016/j.aca.2020.12.006. Epub 2020 Dec 9.
Electrical field-flow fractionation (ElFFF) is a useful separation technique for nanoparticles, however, it has been limited by polarization/electrical double layer formation which reduces an effective field for separation. With an appropriate direct current (DC) applied voltage, sodium carbonate, FL-70, Triton X-100 and acetonitrile were explored as additive substances for preparation of carrier liquid used in normal ElFFF to enhance an amplitude of effective field by their ionic redox-active species, ionic and nonionic surfactant and wide electrochemical potential window nonionic organic solvent properties, respectively. Effective field was indirectly measured in each carrier liquid by investigating retention behavior of polystyrene latex nanoparticles and gold nanoparticles. Effective field improvement was observed in all carrier liquid types (except FL-70) by which the highest effective field existed in 16 μM sodium carbonate at 1.70 V and 0.01% (V/V) Triton X-100 and 50% (V/V) acetonitrile at 1.90 V as compared to deionized water at 1.90 V. In addition, those carrier liquids were applied for separation of 5 nm and 15 nm gold nanoparticles mixture by which Triton X-100 exhibited the best separation resolution (R = 1.11).
电场流场分级（ElFFF）是一种用于纳米颗粒的有用分离技术，然而，它受到极化/双电层形成的限制，这降低了分离的有效场。通过施加适当的直流（DC）电压，研究了碳酸钠、FL-70、Triton X-100 和乙腈作为添加剂物质，用于制备正常 ElFFF 中使用的载液，以通过其离子氧化还原活性物质、离子和非离子表面活性剂以及宽电化学电位窗口非离子有机溶剂特性分别增强有效场的幅度。通过研究聚苯乙烯乳胶纳米颗粒和金纳米颗粒的保留行为，间接测量了每种载液中的有效场。在所有载液类型（FL-70 除外）中都观察到有效场的改善，其中在 16 μM 碳酸钠中在 1.70 V 和 0.01%（V/V）Triton X-100 和 50%（V/V）乙腈中在 1.90 V 下存在最高的有效场，而在 1.90 V 下的去离子水中则存在最高的有效场。此外，还使用这些载液分离 5nm 和 15nm 金纳米颗粒混合物，其中 Triton X-100 表现出最佳的分离分辨率（R=1.11）。
