Kantak Ameya S, Srinivas Merugu, Gale Bruce K
Department of Mechanical Engineering, University of Utah, 50 South Central Campus Drive, MEB Room 2110, Salt Lake City, Utah 84112-9202, USA.
Anal Chem. 2006 Apr 15;78(8):2557-64. doi: 10.1021/ac052127i.
Recent work with cyclical electrical field-flow fractionation systems has shown promise for the technique as a separation and analysis tool, but little is understood about how the carrier composition in the system affects its capabilities. The electrical properties of microscale CyElFFF systems change when the carrier ionic conditions are altered, and it is well known that the effects of increasing ionic strength carriers on retention in normal ElFFF systems are severe. Specifically, retention levels fall significantly. Accordingly, this work seeks to understand the effect that increasing carrier ionic strength in CyElFFF has on nanoparticle retention in the channels. The retention of polystyrene particles in the CyElFFF microsystem is reported at various ionic strengths of ammonium carbonate and at a variety of pH levels. The experiments are compared to the theory of CyElFFF available in the literature. The results indicate that the ionic strength of the carrier has a significant impact on retention and that high ionic strength carrier solutions lead to poor performance of the CyElFFF system. These results have significant impact on the possible uses of the technique and its applications, especially in the biomedical arena.
近期对循环电场流分级系统的研究表明，该技术有望成为一种分离和分析工具，但对于系统中的载体组成如何影响其性能，人们了解甚少。当载体离子条件改变时，微尺度循环电场流分级（CyElFFF）系统的电学性质会发生变化，而且众所周知，在常规电场流分级（ElFFF）系统中，增加离子强度载体对保留率的影响非常严重。具体而言，保留水平会显著下降。因此，本研究旨在了解CyElFFF中载体离子强度增加对通道中纳米颗粒保留率的影响。报告了在不同碳酸铵离子强度和多种pH值水平下，聚苯乙烯颗粒在CyElFFF微系统中的保留情况。将实验结果与文献中可用的CyElFFF理论进行了比较。结果表明，载体的离子强度对保留率有显著影响，高离子强度载体溶液会导致CyElFFF系统性能不佳。这些结果对该技术的可能用途及其应用，尤其是在生物医学领域，具有重大影响。
