Trusk T C, Stein E A
Department of Biology, Marquette University, Milwaukee, WI 53233.
Brain Res. 1988 Jan 12;438(1-2):61-6. doi: 10.1016/0006-8993(88)91323-6.
Brain activity was measured autoradiographically using [1-14C]octanoate (OCTO) as a fast functional tracer in rats receiving either saline, heroin or cocaine. Regional optical densities were normalized to a relative optical density index for comparisons of OCTO labeling between treatment groups. Heroin significantly increased labeling in the dentate gyrus and cocaine increased density in the anterior cingulate cortex, globus pallidus, hippocampus CA3-4, lateral septum, hypothalamus and ventral tegmentum. Heroin and cocaine induced significant, but opposing effects in medial cortex and bed nucleus of the stria terminalis. Both drugs decreased labeling density in the nucleus accumbens and piriform cortex, and increased density in the substantia nigra, subthalamus, medial septum, claustrum, lateral hypothalamus and hippocampus CA2. These results demonstrate the ability of the OCTO method to discriminate the brief metabolic effects of different drug classes, and suggest that heroin and cocaine may activate a common functional system in the brain.
采用放射自显影法，以[1-14C]辛酸（OCTO）作为快速功能示踪剂，对接受生理盐水、海洛因或可卡因的大鼠进行脑活动测量。将区域光密度标准化为相对光密度指数，以比较各治疗组之间的OCTO标记情况。海洛因显著增加了齿状回的标记，而可卡因增加了前扣带回皮质、苍白球、海马CA3-4、外侧隔区、下丘脑和腹侧被盖区的密度。海洛因和可卡因在内侧皮质和终纹床核中诱导出显著但相反的效应。两种药物均降低了伏隔核和梨状皮质的标记密度，并增加了黑质、丘脑底核、内侧隔区、屏状核、外侧下丘脑和海马CA2的密度。这些结果证明了OCTO方法能够区分不同药物类别的短暂代谢效应，并表明海洛因和可卡因可能激活大脑中的一个共同功能系统。
