Kaushik Sharbani, Thungon Phurpa Dema, Goswami Pranab
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43201, United States.
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
ACS Biomater Sci Eng. 2020 Aug 10;6(8):4337-4355. doi: 10.1021/acsbiomaterials.9b01971. Epub 2020 Jul 14.
Enzymes and whole cells serve as the active biological entities in a myriad of applications including bioprocesses, bioanalytics, and bioelectronics. Conserving the natural activity of these functional biological entities during their prolonged use is one of the major goals for validating their practical applications. Silk fibroin (SF) has emerged as a biocompatible material to interface with enzymes as well as whole cells. These biomaterials can be tailored both physically and chemically to create excellent scaffolds of different forms such as fibers, films, and powder for immobilization and stabilization of enzymes. The secondary structures of the SF-protein can be attuned to generate hydrophobic/hydrophilic pockets suitable to create the biocompatible microenvironments. The fibrous nature of the SF protein with a dominant hydrophobic property may also serve as an excellent support for promoting cellular adhesion and growth. This review compiles and discusses the recent literature on the application of SF as a biocompatible material at the interface of enzymes and cells in various fields, including the emerging area of bioelectronics and bioanalytical sciences.
酶和全细胞在包括生物过程、生物分析和生物电子学在内的众多应用中作为活性生物实体发挥作用。在这些功能性生物实体的长期使用过程中保持其天然活性是验证其实际应用的主要目标之一。丝素蛋白（SF）已成为一种可与酶以及全细胞相互作用的生物相容性材料。这些生物材料可以在物理和化学方面进行定制，以创建不同形式（如纤维、薄膜和粉末）的优质支架，用于酶的固定化和稳定化。SF蛋白的二级结构可以进行调节，以产生适合创建生物相容性微环境的疏水/亲水口袋。具有显著疏水特性的SF蛋白的纤维性质也可以作为促进细胞黏附和生长的优良载体。本综述汇编并讨论了近期关于SF作为生物相容性材料在酶和细胞界面在各个领域（包括生物电子学和生物分析科学等新兴领域）应用的文献。
