Nangare Sopan N, Patil Pravin O
H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur-425405, Maharashtra India.
ACS Biomater Sci Eng. 2021 Jan 11;7(1):2-30. doi: 10.1021/acsbiomaterials.0c01203. Epub 2020 Dec 5.
Despite the indisputable benefits and advancement of science, technology, and civilization, early diagnosis of healthcare is still a challenging field for the scientific fraternity. The detection of biomarkers is a crucial attribute of prognosis and diagnosis of disease. Out of numerous techniques, surface plasmon resonance (SPR) bestows countless benefits, including , label-free, and real-time assessment, etc., which authorizes the analysis of molecular binding occurrences between biotransducers and biomarkers. In addition, SPR with low-molecular-weight biomarkers lacks selectivity and sensitivity, which ultimately affects binding kinetics. This, in turn, leads to the remarkable development and implementation of numerous selectivity and sensitivity enhancement methods. Among the various noticeable strategies, because of selectivity and sensitivity enrichment substrate for SPR biosensors, affinity-based nanoarchitectured biotransducers stand out as being the best substitute. The present review elaborates significant advances made in the research based on affinity biotransducers for diagnosis using SPR biosensors for biomarker sensing. Moreover, most recent trends and challenges in designing and application of nanoarchitectured affinity biotransducer-based SPR biosensors for detecting low-concentration biomarkers have been reviewed comprehensively. This present review may assist the scientific fraternity in designing an ultramodern novel SPR approach based on affinity biotransducers, along with improved selectivity and sensitivity of SPR biosensors for and real-time diagnostic applications.
尽管科学、技术和文明带来了无可争议的好处和进步，但医疗保健的早期诊断对科学界来说仍然是一个具有挑战性的领域。生物标志物的检测是疾病预后和诊断的关键属性。在众多技术中，表面等离子体共振（SPR）具有无数优点，包括无标记和实时评估等，这使得能够分析生物传感器与生物标志物之间的分子结合情况。此外，SPR对低分子量生物标志物缺乏选择性和敏感性，这最终会影响结合动力学。反过来，这导致了众多选择性和敏感性增强方法的显著发展和应用。在各种值得注意的策略中，由于SPR生物传感器的选择性和敏感性富集底物，基于亲和力的纳米结构生物传感器脱颖而出，成为最佳替代品。本综述阐述了基于亲和力生物传感器使用SPR生物传感器进行生物标志物传感诊断的研究取得的重大进展。此外，还全面综述了基于纳米结构亲和力生物传感器的SPR生物传感器在检测低浓度生物标志物的设计和应用中的最新趋势和挑战。本综述可能有助于科学界设计一种基于亲和力生物传感器的超现代新型SPR方法，同时提高SPR生物传感器在疾病诊断和实时诊断应用中的选择性和敏感性。
