Yong Xueyong, Hu Qisong, Zhou Ergang, Deng Jianping, Wu Youping
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5072-5081. doi: 10.1021/acsbiomaterials.9b01276. Epub 2019 Sep 13.
Polylactide [PLA, two enantiomers: poly(l-lactide) (PLLA) and poly(d-lactide) (PDLA)] has been widely applied as biomaterials because of its biocompatibility, biodegradability, and good mechanical capacity. However, the chirality of PLA materials has not been intensively explored yet. In the present study, chiral porous poly(high internal-phase emulsion)s (polyHIPEs) derived from enantiopure PLAs were successfully prepared via a HIPE template method. The resulting polyHIPEs show optical activity. More interestingly, the polyHIPEs demonstrate enantioselective release capacity, using cinchona alkaloid and naproxen as the model chiral drugs. Notably, PLLA-based polyHIPE shows enantioselectivity in both the drug-loading step and drug-releasing step, while PDLA-based polyHIPE fails. The interesting finding is essentially different from other chiral polymer materials reported earlier. The cytotoxicity test demonstrates that all the three types of polyHIPEs, PLLA-, PDLA-, and PDLLA-based polyHIPE show biocompatibility; however, their different chirality exerts varying effects on cell growth. Accordingly, special attention should be devoted to the chirality of PLA when used as biomaterials.
聚乳酸[PLA，两种对映体：聚（L-乳酸）（PLLA）和聚（D-乳酸）（PDLA）]因其生物相容性、生物降解性和良好的机械性能而被广泛用作生物材料。然而，PLA材料的手性尚未得到深入研究。在本研究中，通过高内相乳液（HIPE）模板法成功制备了源自对映体纯PLA的手性多孔聚（高内相乳液）（polyHIPEs）。所得的polyHIPEs表现出光学活性。更有趣的是，以金鸡纳生物碱和萘普生作为模型手性药物，polyHIPEs表现出对映选择性释放能力。值得注意的是，基于PLLA的polyHIPE在载药步骤和释药步骤中均表现出对映选择性，而基于PDLA的polyHIPE则不然。这一有趣的发现与先前报道的其他手性聚合物材料有本质区别。细胞毒性测试表明，所有三种类型的polyHIPEs，即基于PLLA、PDLA和PDLLA的polyHIPE均表现出生物相容性；然而，它们不同的手性对细胞生长产生不同的影响。因此，PLA用作生物材料时，应特别关注其手性。
