Department of Biology-Molecular Biology and Genetics Program, Faculty of Science, Firat University, Elazig, Turkey.
Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
Immunopharmacol Immunotoxicol. 2021 Apr;43(2):145-152. doi: 10.1080/08923973.2020.1869255. Epub 2021 Jan 17.
The aim of this study was to evaluate the potential effect of ellagic acid (EA) in the treatment of pancreatic injury. EA has been found to have strong anti-inflammatory, antioxidative, and anticancer properties. The effects of EA on pancreati˜c star cell (PSC) activation and cell functions have been evaluated and it has been shown that it inhibits the activation of basic cell functions and PSCs and it has antidiabetic activity through its effect on β-pancreas cells.
In this work, 36 Wistar albino rats ( = 36, 8 weeks old) were used. Rats were divided to 4 groups and 9 rats were each group. Groups: Group 1: control group; Group 2: EA group; Group 3: carbon tetrachloride (CCl) group; Group 4: EA + CCl group. Animals were decapitated after 8 weeks and their pancreas tissue samples were taken and researched. In pancreas tissue, NF-κB, TNF-α, Nrf-2, VEGF, Bcl-2, caspase-3, and Akt proteins expression ratios were analyzed by western blotting method, CAT activity and GSH levels were determined by spectrophotometer and ROS production was detected by MDA.
In our results, the Nrf-2 and caspase-3 protein expressions, catalase activities and GSH levels increased, TNF-α, NF-κB, Bcl-2, VEGF, and Akt protein expressions and MDA levels reduced in EA + CCl group comparable to the CCl group.
These findings reveal that EA decreases pancreas tissue injury in rats and that EA may also be used as a drug against pancreas tissue injury in the future.
本研究旨在评估鞣花酸（EA）在胰腺损伤治疗中的潜在作用。EA 具有很强的抗炎、抗氧化和抗癌特性。已经评估了 EA 对胰腺星状细胞（PSC）活化和细胞功能的影响，结果表明它抑制了基础细胞功能和 PSCs 的活化，并且通过对β-胰腺细胞的作用具有抗糖尿病活性。
本工作使用 36 只 Wistar 白化大鼠（ = 36，8 周龄）。大鼠分为 4 组，每组 9 只。组：组 1：对照组；组 2：EA 组；组 3：四氯化碳（CCl）组；组 4：EA+CCl 组。8 周后，动物断头并取其胰腺组织样本进行研究。在胰腺组织中，通过 Western 印迹法分析 NF-κB、TNF-α、Nrf-2、VEGF、Bcl-2、caspase-3 和 Akt 蛋白表达比，通过分光光度计测定 CAT 活性和 GSH 水平，并通过 MDA 检测 ROS 产生。
在我们的结果中，与 CCl 组相比，EA+CCl 组的 Nrf-2 和 caspase-3 蛋白表达、过氧化氢酶活性和 GSH 水平增加，TNF-α、NF-κB、Bcl-2、VEGF 和 Akt 蛋白表达和 MDA 水平降低。
这些发现表明 EA 可减轻大鼠胰腺组织损伤，EA 将来可能也可作为治疗胰腺组织损伤的药物。
