Aslan Abdullah, Gok Ozlem, Beyaz Seda, Arslan Emre, Erman Orhan, Ağca Can Ali
Department of Biology-Molecular Biology and Genetics Program, Faculty of Science, University of Firat, Elazığ, Turkey.
Department of Biology, Faculty of Science, University of Firat, Elazığ, Turkey.
J Food Biochem. 2020 Jun;44(6):e13217. doi: 10.1111/jfbc.13217. Epub 2020 Apr 6.
The aim of this study was to investigate the neuroprotective role of ellagic acid (EA) on CCl -induced brain injury in rats. In this study, the rats were divided into four groups. Groups: (1) Control group; (2) EA group; (3) CCl group; (4) EA + CCl group. In brain tissue, tumor necrosis factor-α (TNF-α), nuclear factor kappa b (NF-kB), cyclooxygenase-2 (COX-2), nuclear erythroid related factor 2 (Nrf-2), cysteine-aspartic acid protease (caspase-3), VEGF (vascular endothelial growth factor) and B-cell lymphoma-2 (bcl-2) protein expression levels were analyzed by western blotting. MDA (malondialdehyde), catalase enzyme activity (CAT) and glutathione (GSH) analysis were determined by spectrophotometer. In our findings, EA ameliorated Nrf-2 and caspase-3 protein expression levels, GSH and catalase activities, NF-kB, TNF-α, VEGF, Bcl-2, COX-2 protein expression levels and MDA levels in CCl intoxicated rats. These results suggest that EA demonstrated the neuroprotective effect on CCl -induced brain damage in rats. PRACTICAL APPLICATIONS: Ellagic acid has different biological activities, these are; antioxidant, anti-inflammatory, antidepressant, antifibrosis, anticancer, neuroprotective and hepatoprotective. For example it was reported that EA protects the cells against DNA injury induced by free radicals and it can prevent the traumatic brain injury. These results obtained from this study reveals that EA has a protective effect against rat brain damage and it may be used as an alternative drugs for the brain injury treatment in future.
本研究旨在探讨鞣花酸（EA）对四氯化碳（CCl）诱导的大鼠脑损伤的神经保护作用。在本研究中，将大鼠分为四组。分组：（1）对照组；（2）EA组；（3）CCl组；（4）EA + CCl组。通过蛋白质免疫印迹法分析脑组织中肿瘤坏死因子-α（TNF-α）、核因子κB（NF-kB）、环氧化酶-2（COX-2）、核红细胞相关因子2（Nrf-2）、半胱天冬酶-3（caspase-3）、血管内皮生长因子（VEGF）和B细胞淋巴瘤-2（bcl-2）蛋白表达水平。通过分光光度计测定丙二醛（MDA）、过氧化氢酶活性（CAT）和谷胱甘肽（GSH）。在我们的研究结果中，EA改善了CCl中毒大鼠的Nrf-2和caspase-3蛋白表达水平、GSH和过氧化氢酶活性、NF-kB、TNF-α、VEGF、Bcl-2、COX-2蛋白表达水平以及MDA水平。这些结果表明，EA对CCl诱导的大鼠脑损伤具有神经保护作用。实际应用：鞣花酸具有不同的生物活性，这些活性包括抗氧化、抗炎、抗抑郁、抗纤维化、抗癌、神经保护和肝保护。例如，据报道EA可保护细胞免受自由基诱导的DNA损伤，并且它可以预防创伤性脑损伤。本研究获得的这些结果表明，EA对大鼠脑损伤具有保护作用，并且在未来可能用作脑损伤治疗的替代药物。
