Morigaki Kenichi
Biosignal Research Center, Kobe University, 1-1 Rokkodaicho, Nada, Kobe, 657-8501, Japan.
Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, Nada, Kobe, 657-8501, Japan.
Anal Sci. 2021 May 10;37(5):683-689. doi: 10.2116/analsci.20SCR10. Epub 2021 Jan 15.
Biological membranes composed of a lipid bilayer and associated proteins work as a platform for highly selective and sensitive detection in nature. Substrate-supported lipid bilayers (SLBs) are a model system of the biological membrane that are mechanically stable, accessible to highly sensitive analytical techniques, and amenable to micro-fabrication, such as patterning. The surface of SLBs can effectively suppress the non-specific binding of proteins, and enhance selective detection by specific interactions. These features render SLBs highly attractive for the development of devices that utilize artificially mimicked cellular functions. Furthermore, SLBs can be combined with nanoscopic spaces, such as nano-channels and nano-pores, that can reduce the detection volume and suppress the non-specific background noise, enhancing the signal-to-background noise (S/B) ratio. SLBs therefore provide promising platforms for a wide range of biomedical and environmental analyses.
由脂质双层和相关蛋白质组成的生物膜在自然界中作为高度选择性和灵敏检测的平台发挥作用。底物支撑脂质双层（SLBs）是生物膜的一种模型系统，具有机械稳定性，可采用高灵敏度分析技术进行检测，并且适合进行微加工，如图案化处理。SLBs的表面能够有效抑制蛋白质的非特异性结合，并通过特异性相互作用增强选择性检测。这些特性使得SLBs对于开发利用人工模拟细胞功能的设备极具吸引力。此外，SLBs可以与纳米级空间（如纳米通道和纳米孔）相结合，这能够减小检测体积并抑制非特异性背景噪声，提高信噪比（S/B）。因此，SLBs为广泛的生物医学和环境分析提供了有前景的平台。
