Pomothy Judit Mercédesz, Pászti-Gere Erzsébet, Barna Réka Fanni, Prokoly Dorottya, Jerzsele Ákos
Department of Pharmacology and Toxicology, University of Veterinary Medicine, 1078 Budapest, Hungary.
Department of Physiology and Biochemistry, University of Veterinary Medicine, 1078 Budapest, Hungary.
Oxid Med Cell Longev. 2020 Dec 8;2020:3854247. doi: 10.1155/2020/3854247. eCollection 2020.
The effect of fermented wheat germ extract (FWGE) () was evaluated with cotreatments with deoxynivalenol (DON) and T-2 toxin (T-2). These mycotoxins are produced by mold species. The effects of FWGE on IPEC-J2 with DON and T-2 have not been studied until now. The IPEC-J2 porcine, nontumorigenic cell line was selected to investigate the outcome of the individually and simultaneously added compounds, as it has -like properties. The cells were treated for 24 h with the selected solutions; then, the IPEC-J2 cells were allowed to regenerate in a culture medium for an additional 24 h. In our results, DON and T-2 significantly increased the adverse impacts on cell viability and integrity of the cell monolayer. To elucidate the extent of oxidative stress, extracellular HO concentrations and intracellular reactive oxygen species (ROS) were measured. FWGE appeared to be beneficial to IPEC-J2 cells given the separately and significantly decreased ROS levels. 1% and 2% FWGE could significantly reduce mycotoxin-induced oxidative stress. In conclusion, the results demonstrate that FWGE exerted protective effects to counteract the oxidative stress-provoking properties of applied fusariotoxins in the nontumorigenic IPEC-J2 cell line.
通过与脱氧雪腐镰刀菌烯醇（DON）和T-2毒素（T-2）共同处理来评估发酵小麦胚芽提取物（FWGE）的效果。这些霉菌毒素由霉菌种类产生。到目前为止，尚未研究FWGE对IPEC-J2细胞与DON和T-2共同作用的影响。选择IPEC-J2猪非致瘤细胞系来研究单独添加和同时添加化合物的结果，因为它具有类似的特性。用选定的溶液处理细胞24小时；然后，使IPEC-J2细胞在培养基中再培养24小时以进行再生。在我们的结果中，DON和T-2显著增加了对细胞活力和细胞单层完整性的不利影响。为了阐明氧化应激的程度，测量了细胞外HO浓度和细胞内活性氧（ROS）。鉴于FWGE分别显著降低了ROS水平，其似乎对IPEC-J2细胞有益。1%和2%的FWGE可显著降低霉菌毒素诱导的氧化应激。总之，结果表明FWGE发挥了保护作用，以抵消应用的镰刀菌毒素在非致瘤性IPEC-J2细胞系中引发氧化应激的特性。
