Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, H-1078 Budapest, Hungary.
Department of Physiology and Biochemistry, University of Veterinary Medicine Budapest, H-1078 Budapest, Hungary.
Mediators Inflamm. 2020 Dec 22;2020:8880651. doi: 10.1155/2020/8880651. eCollection 2020.
Mycotoxin contamination in feedstuffs is a worldwide problem that causes serious health issues both in humans and animals, and it contributes to serious economic losses. Deoxynivalenol (DON) and T-2 toxin (T-2) are major trichothecene mycotoxins and are known to challenge mainly intestinal barrier functions. Polyphenolic rosmarinic acid (RA) appeared to have antioxidant and anti-inflammatory properties . The aim of this study was to investigate protective effects of RA against DON and T-2 or combined mycotoxin-induced intestinal damage in nontumorigenic porcine cell line, IPEC-J2. It was ascertained that simultaneous treatment of DON and T-2 (DT2: 1 mol/L DON + 5 nmol/L T - 2) for 48 h and 72 h reduced transepithelial electrical resistance of cell monolayer, which was restored by 50 mol/L RA application. It was also found that DT2 for 48 h and 72 h could induce oxidative stress and elevate interleukin-6 (IL-6) and interleukin-8 (IL-8) levels significantly, which were alleviated by the administration of RA. DT2 administration contributed to the redistribution of claudin-1; however, occludin membranous localization was not altered by combined mycotoxin treatment. In conclusion, beneficial effect of RA was exerted on DT2-deteriorated cell monolayer integrity and on the perturbated redox status of IPEC-J2 cells.
饲料中的霉菌毒素污染是一个全球性问题，它会给人类和动物的健康带来严重问题，并导致严重的经济损失。脱氧雪腐镰刀菌烯醇（DON）和 T-2 毒素（T-2）是主要的单端孢霉烯族毒素，已知主要挑战肠道屏障功能。多酚迷迭香酸（RA）具有抗氧化和抗炎特性。本研究旨在研究 RA 对 DON 和 T-2 或联合霉菌毒素诱导的非致瘤猪细胞系 IPEC-J2 肠道损伤的保护作用。结果表明，DON 和 T-2（DT2：1mol/L DON + 5nmol/L T-2）同时处理 48h 和 72h 降低了细胞单层的跨上皮电阻，而 50μmol/L RA 的应用可恢复其电阻。还发现 DT2 处理 48h 和 72h 可诱导氧化应激并显著增加白细胞介素-6（IL-6）和白细胞介素-8（IL-8）水平，而 RA 的给药可减轻这些变化。DT2 处理导致 Claudin-1 重新分布；然而，联合霉菌毒素处理并未改变紧密连接蛋白的膜定位。总之，RA 对 DT2 恶化的细胞单层完整性和 IPEC-J2 细胞的氧化还原状态紊乱具有有益作用。
